Antiproliferation effects of oridonin on HL-60 cells
Antiproliferation effects of oridonin on HL-60 cells
复制标题
DOI：
10.1007/s00277-004-0919-y
复制
发表时间：
2004-08
影响因子：
3.5
通讯作者：
Jia-Jun Liu;Xinyao Wu;J. Peng;X. Pan;Huiling Lu
中科院分区：
文献类型：
--
作者：
Jia-Jun Liu;Xinyao Wu;J. Peng;X. Pan;Huiling Lu
本文研究了冬凌草甲素对白血病HL-60细胞的增殖抑制作用及对端粒酶活性的抑制作用。用不同浓度的冬凌草甲素处理HL-60细胞。MTT法检测细胞生长抑制率。流式细胞仪检测细胞凋亡率。瑞氏染色观察细胞凋亡形态。采用逆转录聚合酶链反应（RT-PCR）和PCR-酶联免疫吸附法（ELISA）检测细胞凋亡前后hTERTmRNA表达和端粒酶活性。冬凌草甲素（> 8 μmol/l）可明显抑制HL-60细胞的生长，并引起细胞凋亡。抑制作用呈时间依赖性和剂量依赖性。瑞氏染色可见细胞凋亡的形态学改变，尤其是在冬凌草甲素作用48-60 h后，细胞核染色质浓缩、核碎裂。冬凌草甲素可抑制HL-60细胞端粒酶活性和hTERTmRNA表达。冬凌草甲素能下调HL-60细胞hTERTmRNA表达，降低端粒酶活性，对HL-60细胞有明显的体外抗增殖和诱导凋亡作用。
The antiproliferation effects and inhibition of telomerase activity of oridonin on leukemic HL-60 cells were studied. HL-60 cells in culture medium were treated with different concentrations of oridonin. The inhibitory rate of the cells was measured by MTT assay. Cell apoptotic rate was detected by flow cytometry (FCM). Morphology of cell apoptosis was observed by Wright’s stain. Reverse transcriptase polymerase chain reaction (RT-PCR) and PCR–enzyme-linked immunosorbent assay (ELISA) were used to detect hTERT mRNA expression and telomerase activity before and after apoptosis. Oridonin (over 8 μmol/l) could inhibit the growth of HL-60 cells and cause apoptosis significantly. The suppression was in both time-dependent and dose-dependent manner. Marked morphological changes of cell apoptosis including condensation of chromatin and nuclear fragmentation were observed clearly by Wright’s stain especially after the cells were treated 48–60 h by oridonin. The expression of hTERT mRNA as well as activity of telomerase were decreased concurrently by treatment with oridonin in HL-60 cells. Oridonin can downregulate the hTERT mRNA expression and decrease the telomerase activity of HL-60 cells; it has apparent antiproliferation and apoptosis-inducing effects on HL-60 cells in vitro.