PHOSPHATIDYLINOSITOL 3-KINASE ENCODED BY YEAST VPS34 GENE ESSENTIAL FOR PROTEIN SORTING
PHOSPHATIDYLINOSITOL 3-KINASE ENCODED BY YEAST VPS34 GENE ESSENTIAL FOR PROTEIN SORTING
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DOI：
10.1126/science.8385367
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发表时间：
1993-04-02
期刊：
影响因子：
56.9
通讯作者：
EMR, SD
中科院分区：
文献类型：
--
作者：
SCHU, PV;TAKEGAWA, K;EMR, SD
VPS34 基因产物 (Vps34p) 是对酿酒酵母溶酶体样液泡进行蛋白质分选所必需的。 Vps34p 与牛磷脂酰肌醇 (PI) 3-激酶的催化亚基 [PI 3-激酶的 110 千道尔顿 (p110) 亚基] 具有显着的序列相似性，已知后者与激活的细胞表面受体酪氨酸激酶相互作用。删除 VPS34 基因或携带 vps34 点突变的酵母菌株缺乏可检测的 PI 3 激酶活性，并且在液泡蛋白分选方面表现出严重缺陷。 Vps34p 的过度表达导致 PI 3 激酶活性增加，并且该活性通过 Vps34p 抗血清特异性沉淀。 VPS34 编码酵母 PI 3 激酶，这种酶似乎可以调节细胞内蛋白质运输决策。
The VPS34 gene product (Vps34p) is required for protein sorting to the lysosome-like vacuole of the yeast Saccharomyces cerevisiae. Vps34p shares significant sequence similarity with the catalytic subunit of bovine phosphatidylinositol (PI) 3-kinase [the 110-kilodalton (p110) subunit of PI 3-kinase], which is known to interact with activated cell surface receptor tyrosine kinases. Yeast strains deleted for the VPS34 gene or carrying vps34 point mutations lacked detectable PI 3-kinase activity and exhibited severe defects in vacuolar protein sorting. Overexpression of Vps34p resulted in an increase in PI 3-kinase activity, and this activity was specifically precipitated with antisera to Vps34p. VPS34 encodes a yeast PI 3-kinase, and this enzyme appears to regulate intracellular protein trafficking decisions.