Immunocytochemical localization of nuclear estrogen receptors and progestin receptors within the rat dorsal raphe nucleus
Immunocytochemical localization of nuclear estrogen receptors and progestin receptors within the rat dorsal raphe nucleus
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DOI：
10.1002/(sici)1096-9861(19980216)391:3
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发表时间：
1998-02-16
影响因子：
2.5
通讯作者：
Mcewen, BS
中科院分区：
文献类型：
--
作者：
Alves, SE;Weiland, NG;Mcewen, BS
雌二醇和孕酮调节中枢孕酮能活动;然而，作用机制尚不清楚。最近，雌二醇诱导的β-羟色胺受体（PR）已定位于雌性猕猴中缝背核中的大多数5-羟色胺（5-HT）神经元内（DRN; Bethea [1994] Neuroendocrinology 60：50-61）。在本研究中，我们调查雌激素受体（ER）和/或PR是否存在于5-HT和/或非5-HT细胞在雌性和雄性大鼠DRN和雌二醇治疗是否改变这些受体的表达。切除年轻成年雌性和雄性Sprague-Dawley大鼠的性腺，1周后，一半动物接受雌二醇-17 β的皮下硅橡胶植入物。2天后用丙烯醛和多聚甲醛经心脏灌注动物，并通过使用PR抗体或ER α抗体在相邻切片上进行连续双标记免疫细胞化学。随后是5-HT合成酶色氨酸羟化酶（TPH）或星形胶质细胞标记物胶质细胞酸性蛋白（GFAP）的抗体。细胞核ER或PR的免疫反应性（IR）内确定的大鼠DRN在特定区域的分布在两种性别。在两种性别或治疗中均未观察到细胞核ER-IR或PR-IR与细胞质TPH-IR或GFAP-IR的共定位;表明类固醇靶细胞既不是5-HT神经元也不是星形胶质细胞。雌鼠DRN内PR阳性神经元的数量比雄鼠多30%（P < 0.05），但ER阳性神经元的数量在性别上无差异，雌鼠暴露2 d后，DRN内ER阳性神经元的数量减少，PR阳性神经元的数量显著增加（P < 0.005）。总的来说，这些研究结果表明，存在noncervionergic细胞，含有核ER或PR的女性和男性大鼠DRN内，包括雌二醇诱导的PR。这些发现表明猕猴和大鼠之间卵巢类固醇对5-HT活性的调节存在种属差异，可能是通过大鼠大脑中的局部神经元进行的跨突触调节。(C)1998 Wiley-Liss，Inc.
Estradiol and progesterone modulate central serotonergic activity; however, the mechanism(s) of action remain unclear. Recently estradiol-induced progestin receptors (PRs) have been localized within the majority of serotonin (5-HT) neurons in the female macaque dorsal raphe nucleus (DRN; Bethea [1994] Neuroendocrinology 60:50-61). In the present study, we investigated whether estrogen receptors (ERs) and/or PRs exist within 5-HT and/or non-5-HT cells in the female and male rat DRN and whether estradiol treatment alters the expression of these receptors. Young adult female and male Sprague-Dawley rats were gonadectomized, and 1 week later, half of the animals received a subcutaneous Silastic implant of estradiol-17 beta. Animals were transcardially perfused 2 days later with acrolein and paraformaldehyde, and sequential dual-label immunocytochemistry was performed on adjacent sections by using either a PR antibody or an ER alpha antibody. This was followed by an antibody to either the 5-HT-synthesizing enzyme, tryptophan hydroxylase (TPH), or to the astrocytic marker, glial fibrillary acidic protein (GFAP). Cells containing immunoreactivity (ir) for nuclear ERs or PRs were identified within the rat DRN in a region-specific distribution in both sexes. No colocalization of nuclear ER-ir or PR-ir with cytoplasmic TPH-ir or GFAP-ir was observed in either sex or treatment; indicating that the steroid target cells are neither 5-HT neurons nor astrocytes. Females were found to have approximately 30% more PR-labeled cells compared with males throughout the DRN (P < 0.05), but no sex difference was detected in the number of neurons demonstrating ER-ir. In both sexes, 2 days of estradiol exposure decreased the number of cells with ER-ir, whereas it greatly increased the number of cells containing PR-ir in several DRN regions (P < 0.005). Collectively, these findings demonstrate the existence of nonserotonergic cells that-contain nuclear ERs or PRs within the female and male rat DRN, including estradiol-inducible PRs. These findings point to a species difference in ovarian steroid regulation of 5-HT activity between the macaque and the rat, perhaps transsynaptically via local neurons in the rat brain. (C) 1998 Wiley-Liss, Inc.