Spatiotemporal profiles of dental pulp nociception in rat cerebral cortex: An optical imaging study
Spatiotemporal profiles of dental pulp nociception in rat cerebral cortex: An optical imaging study
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DOI：
10.1002/cne.23692
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发表时间：
2015-06
影响因子：
2.5
通讯作者：
H. Nakamura;Risako Kato;T. Shirakawa;N. Koshikawa;Masayuki Kobayashi
中科院分区：
文献类型：
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作者：
H. Nakamura;Risako Kato;T. Shirakawa;N. Koshikawa;Masayuki Kobayashi
躯体感觉在初级躯体感觉皮层（S1）和次级躯体感觉皮层（S2）中进行地形组织，这有助于识别接受感觉输入的区域。然而，它仍然是未知的，如何从口腔区域的躯体感觉输入，特别是来自牙齿的伤害性输入，在躯体感觉皮层处理。我们进行了在体光学成像，并确定了精确的皮质区域响应于电刺激的大鼠上颌骨和下颌牙髓。电刺激下颌切牙牙髓诱发的神经兴奋在两个领域：最rostroventral部分的S1，和腹侧部分的S2尾侧大脑中动脉。上颌切牙牙髓刺激最初只在腹侧部分的S2引起的反应，虽然后来的最大反应也观察到在S1类似的下颌切牙刺激反应。上颌和下颌磨牙牙髓反应区位于最腹侧S2，其中一部分在组织学上被分类为岛状口腔区（IOR）。在初始反应方面，上颌切牙和磨牙刺激诱导了下颌牙髓反应区吻侧的S2/IOR兴奋。与空间分离的初始反应相反，在S1和S2/IOR中，对下颌骨和上颌骨中的切牙和磨牙的最大兴奋区域重叠。多电极细胞外记录支持的特征性定位的S2/IOR神经元响应下颌和上颌磨牙牙髓刺激。初始反应和最大反应的离散和重叠的空间分布可以表征皮层对牙痛伤害性信息的处理。神经学比较杂志523：1162-1174，2015。© 2015 Wiley Periodicals，Inc.
Somatosensation is topographically organized in the primary (S1) and secondary somatosensory cortex (S2), which contributes to identify the region receiving sensory inputs. However, it is still unknown how somatosensory inputs from the oral region, especially nociceptive inputs from the teeth, are processed in the somatosensory cortex. We performed in vivo optical imaging and identified the precise cortical regions responding to electrical stimulation of the maxillary and mandibular dental pulp in rats. Electrical stimulation of the mandibular incisor pulp evoked neural excitation in two areas: the most rostroventral part of S1, and the ventral part of S2 caudal to the middle cerebral artery. Maxillary incisor pulp stimulation initially evoked responses only in the ventral part of S2, although later maximum responses were also observed in S1 similar to mandibular incisor stimulation responses. The maxillary and mandibular molar pulp‐responding regions were located in the most ventral S2, a part of which was histologically classified as the insular oral region (IOR). In terms of the initial responses, maxillary incisor and molar stimulation induced excitation in the S2/IOR rostral to the mandibular dental pulp‐responding region. Contrary to the spatially segregated initial responses, the maximum excitatory areas responding to both incisors and molars in the mandible and maxilla overlapped in S1 and the S2/IOR. Multielectrode extracellular recording supported the characteristic localization of S2/IOR neurons responding to mandibular and maxillary molar pulp stimulation. The discrete and overlapped spatial profiles of initial and maximum responses, respectively, may characterize nociceptive information processing of dental pain in the cortex. J. Comp. Neurol. 523:1162–1174, 2015. © 2015 Wiley Periodicals, Inc.