25-Hydroxyvitamin D assays: Potential interference from other circulating vitamin D metabolites
25-Hydroxyvitamin D assays: Potential interference from other circulating vitamin D metabolites
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DOI：
10.1016/j.jsbmb.2015.12.018
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发表时间：
2016-11-01
影响因子：
4.1
通讯作者：
Sempos, C.
中科院分区：
文献类型：
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作者：
Carter, G. D.;Jones, J. C.;Sempos, C.
25-羟基维生素D（25-OHD）的维生素D外部质量评估计划（DEQAS）在53个国家使用26种不同的方法或方法变体（2014年10月）约有1100名参与者。2015年4月，该计划扩大到包括24，25-二羟维生素D。(24，25（OH）（2）D）。自2013年以来，25-OHD方案一直以NIST参考测量程序（RMP）指定的值为基础。DEQAS在常规实验室环境中评估25-OHD方法的准确性（偏倚）和特异性方面具有独特的优势。已知其他维生素D代谢物会干扰25-OHD测定，DEQAS已分发加标3-epi-25-OHD 3（52.4 nmol/L）、24 R，25（OH）（2）D-3（14.4 nmol/L）和245，25（OH）（2）D-3（57.9 nmol/L）的样本。3-差向异构体在竞争性蛋白结合试验中显示出56%的交叉反应性，但在任何基于抗体的方法中均未检测到。并非所有HPLC/UV或LC-MS/MS方法都能够从25-OHD 3中分离3-表-25-OHD 3，因此高估了总25-OHD：24 R，25（OH）（2）D-3（245，25（OH）（2）D-3）的交叉反应性范围为
The vitamin D External Quality Assessment Scheme (DEQAS) for 25-hydroxyvitamin D (25-OHD) has approximately 1100 participants in 53 countries using 26 different methods or variants of methods (October 2014). In April 2015, the scheme was extended to cover 24,25-dihydroxyvitamin D. (24,25 (OH)(2)D). Since 2013, the 25-OHD scheme has been accuracy-based with values assigned by the NIST reference measurement procedure (RMP). DEQAS is uniquely placed to assess the accuracy (bias) and specificity of 25-OHD methods in a routine laboratory setting. Other vitamin D metabolites are known to interfere in 25-OHD assays and DEQAS has distributed samples spiked with 3-epi-25-OHD3 (52.4 nmol/L), 24R,25(OH)(2)D-3 (14.4 nmol/L) and 245,25(OH)(2)D-3 (57.9 nmol/L). The 3-epimer showed a cross reactivity of 56% in a competitive protein binding assay but was not detected in any antibody-based methods. Not all HPLC/UV or LC-MS/MS methods were able to resolve 3-epi-25-OHD3 from 25-OHD3 and thus overestimated total 25-OHD:The cross reactivity of 24R,25(OH)(2)D-3 (245,25(OH)(2)D-3) ranged from