Neurogenesis within the juvenile zebra finch telencephalic ventricular zone: A map of proliferative activity
Neurogenesis within the juvenile zebra finch telencephalic ventricular zone: A map of proliferative activity
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DOI：
10.1002/cne.20352
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发表时间：
2005-01-03
影响因子：
2.5
通讯作者：
Bottjer, SW
中科院分区：
文献类型：
--
作者：
DeWulf, V;Bottjer, SW
在幼年雄性鸣禽的心室区（VZ）内局部区域的细胞增殖增加可能包含祖细胞，这些祖细胞产生歌曲控制神经元，从而有助于构建对歌曲学习很重要的脑区。本研究的目的是检查整个端脑VZ的幼鸟细胞分裂的水平。对30日龄的雄性和雌性斑胸草雀给予单次脉冲的[H-3]胸苷，2小时后将鸟杀死。VZ被分为整个前-后和背-腹神经轴段，并在每个细分内测量胸苷标记水平。通过将VZ细分为片段，我们能够构建整个端脑VZ的增殖“地图”，从而使我们能够比较男性和女性之间VZ相应位置内的有丝分裂活性水平。我们的图谱揭示了两个主要发现：（1）在整个VZ中，幼年雄性和雌性的增殖在空间上存在差异，表明有丝分裂活性在整个轴突中受到不同的调节;（2）在30日龄的鸟类中存在增殖的性别差异，但受到高度限制。最强大的性二型发生在腹侧方面的VZ在吻侧水平的歌曲控制核X区，与男性表现出分裂细胞的数量增加与女性相比。这一结果提出了一种可能性，即男性的X区神经元来源于邻近VZ内的定向祖细胞，这些祖细胞非常接近该核。(C)2004 Wiley-Liss，Inc.
Localized regions of increased cellular proliferation within the ventricular zone (VZ) of juvenile male songbirds may contain progenitor cells that give rise to song-control neurons and, thereby, contribute to the construction of brain areas important for song learning. The purpose of this study was to examine levels of cell division throughout the telencephalic VZ of juvenile birds. A single pulse of [H-3]thymidine was administered to 30-day male and female zebra finches, and the birds were killed 2 hours later. The VZ was divided into segments throughout the entire anterior-posterior and dorsal-ventral neuraxes, and levels of thymidine labeling were measured within each subdivision. By subdividing the VZ into segments, we were able to construct a "map" of proliferation throughout the telencephalic VZ, thereby allowing us to compare levels of mitotic activity within corresponding locations of the VZ between males and females. Our map revealed two major findings: (1) proliferation in both juvenile males and females was spatially differentiated throughout the VZ, suggesting that mitotic activity is differentially regulated across the neuraxis; (2) sex differences in proliferation were present in 30-day-old birds, but were highly restricted. The most robust sexual dimorphism occurred within the ventral aspect of the VZ at rostral levels of the song-control nucleus Area X, with males demonstrating an increased number of dividing cells compared with females. This result raises the possibility that Area X neurons in males are derived from committed progenitors within the adjacent VZ in close proximity to this nucleus. (C) 2004 Wiley-Liss, Inc.