Improved luciferase tagging system for Listeria monocytogenes allows real-time monitoring in vivo and in vitro
Improved luciferase tagging system for Listeria monocytogenes allows real-time monitoring in vivo and in vitro
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DOI：
10.1128/aem.02940-06
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发表时间：
2007-05-01
影响因子：
4.4
通讯作者：
Gahan, Cormac G. M.
中科院分区：
文献类型：
--
作者：
Riedel, Christian U.;Monk, Ian R.;Gahan, Cormac G. M.
通过构建一个高活性的组成型启动子，建立了一个改进的单增李斯特菌荧光素酶标记系统。该构建体在肉汤中的活性比测试的任何天然启动子高100倍，并允许对lux标记的L.在食品中的单核细胞增多症，在鼠感染和肿瘤靶向研究。
An improved system for luciferase tagging Listeria monocytogenes was developed by constructing a highly active, constitutive promoter. This construct gave 100-fold-higher activity in broth than any native promoter tested and allowed for imaging of lux-tagged L. monocytogenes in food products, during murine infections, and in tumor targeting studies.