Systematic changes in gene expression patterns following adaptive evolution in yeast
Systematic changes in gene expression patterns following adaptive evolution in yeast
复制标题
DOI：
10.1073/pnas.96.17.9721
复制
发表时间：
1999-08-17
影响因子：
11.1
通讯作者：
Rosenzweig, RF
中科院分区：
文献类型：
--
作者：
Ferea, TL;Botstein, D;Rosenzweig, RF
在未最佳适应的条件下培养酿酒酵母群体许多代，选择更适合的遗传变体，这种简单的实验设计提供了自然选择下适应性进化的易处理模型。首先，从克隆的创始种群开始，在葡萄糖限制的恒化器中连续有氧生长超过 250 代后，从三个独立的培养物中获得独立进化的菌株。 DNA 微阵列用于比较进化菌株和亲本菌株的全基因组基因表达模式。数百个基因被发现在进化菌株中表达显着改变，其中许多基因在所有三个进化菌株中表现出相似的表达改变，在三个进化菌株中表达改变的基因包括参与糖酵解、三羧酸循环、氧化磷酸化和代谢物转运的基因。这些结果与生理学观察结果一致，并表明通过改变中枢代谢的调节，使得更少的葡萄糖被发酵，更多的葡萄糖被完全氧化，从而获得增强的健康。
Culturing a population of Saccharomyces cerevisiae for many generations under conditions to which it is not optimally adapted selects for fitter genetic variants, This simple experimental design provides a tractable model of adaptive evolution under natural selection. Beginning,vith a clonal, founding population, independently evolved strains were obtained from three independent cultures after continuous aerobic growth in glucose-limited chemostats for more than 250 generations. DNA microarrays were used to compare genome-wide patterns of gene expression in the evolved strains and the parental strain. Several hundred genes were found to have significantly altered expression in the evolved strains, Many of these genes showed similar alterations in their expression in all three evolved strains, Genes with altered expression in the three evolved strains included genes involved in glycolysis, the tricarboxylic acid cycle, oxidative phosphorylation, and metabolite transport. These results are consistent with physiological observations and indicate that increased fitness is acquired by altering regulation of central metabolism such that less glucose is fermented and more glucose is completely oxidized.