Lower Extent but Similar Rhythm of Osteogenic Behavior in hBMSCs Cultured on Nanofibrous Scaffolds versus Induced with Osteogenic Supplement
Lower Extent but Similar Rhythm of Osteogenic Behavior in hBMSCs Cultured on Nanofibrous Scaffolds versus Induced with Osteogenic Supplement
复制标题
与用成骨补充剂诱导的相比，在纳米纤维支架上培养的 hBMSC 成骨行为的程度较低，但相似的节律。
DOI：
10.1021/nn402118s
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发表时间：
2013-08-01
期刊：
影响因子：
17.1
通讯作者：
Deng, Xuliang
中科院分区：
文献类型：
--
作者：
Liu, Wentao;Wei, Yan;Deng, Xuliang
生物材料的纳米形貌线索对间充质干细胞的成骨分化产生了强大的影响，因为它们具有小生境模仿特征。然而，通过表面纳米形貌确定细胞谱系的生物学机制尚未明确阐明。在这里，我们探讨了人骨髓间充质干细胞（hBMSCs）的成骨行为的聚乳酸纳米纤维与不同的方向和监测的动态变化，全球基因表达触发的地形线索。成骨标记基因的RT-PCR分析和ALP活性测定表明，与对齐的纳米纤维相比，在随机纳米纤维上培养的hBMSCs显示出增强的成骨特异性命运。微阵列分析表明，在随机纳米纤维上培养的hBMSCs和用成骨补充剂（OS）诱导的hBMSCs之间的基因表达模式存在类似的时间变化。然而，纤维支架上的成骨分化程度远低于化学OS驱动的成骨分化程度。深入的通路分析显示，黏着斑激酶、TGF-β、Wnt和MAPK通路参与了随机纳米纤维上hBMSCs成骨分化的激活。这些发现表明，与OS条件相比，随机纳米纤维诱导的动态细胞行为的程度较低，但相似的节奏，并且机械转导可以触发hBMSCs的非特异性和多层次反应。这项研究提供了深入了解的调控骨生成的指导下，基质表面。
Nanotopographic cues from biomaterials exert powerful effects on the osteogenic differentiation of mesenchymal stem cells because of their niche-mimicking features. However, the biological mechanisms underlying cell lineage determination by surface nanotopography have not been clearly elucidated. Here, we explored the osteogenic behavior of human bone marrow mesenchymal stem cells (hBMSCs) on poly-l-lactide nanofibers with different orientations and monitored the dynamic changes in global gene expression triggered by topographical cues. RT-PCR analysis of osteogenic marker genes and ALP activity assays demonstrated that hBMSCs cultured on random nanofibers showed enhanced osteogenic-specific fate compared with those on aligned nanofibers. Microarray analysis demonstrated a similar temporal change in gene expression patterns between hBMSCs cultured on random nanofibers and those induced with an osteogenic supplement (OS). However, the extent of osteogenic differentiation on the fibrous scaffold was much lower than that driven by chemical OS. In-depth pathway analysis revealed that focal adhesion kinase, TGF-β, Wnt, and MAPK pathways were involved in the activation of osteogenic differentiation in hBMSCs on random nanofibers. These findings suggested that a lower extent but similar rhythm of dynamic cellular behavior was induced on random nanofibers when compared with the OS condition and that mechanotransduction could trigger nonspecific and multilevel responses in hBMSCs. This study provides insight into the regulation of osteogenesis directed by substratum surfaces.