Effect of endurance training on the phospholipid content of skeletal muscles in the rat
Effect of endurance training on the phospholipid content of skeletal muscles in the rat
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DOI：
10.1007/s004210050532
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发表时间：
1999-04-01
期刊：
影响因子：
--
通讯作者：
Glatz, JFC
中科院分区：
文献类型：
--
作者：
Górski, J;Zendzian-Piotrowska, M;Glatz, JFC
只有很少的数据。训练对骨骼肌磷脂代谢的影响。本研究的目的是检查6周耐力训练对大鼠不同骨骼肌（腓肠肌、比目鱼肌和横膈膜的白色和红色部分）中特定磷脂组分的含量以及对血传[C-14]-棕榈酸掺入磷脂的影响。从肌肉中提取脂质，并使用薄层色谱法分离成以下馏分：鞘磷脂、磷脂酰胆碱、磷脂酰丝氨酸、磷脂酰肌醇、磷脂酰乙醇胺、心磷脂和中性脂质（该馏分主要由三酰甘油组成）。结果发现，训练不影响比目鱼肌中任何磷脂组分的含量。增加白色腓肠肌鞘磷脂、红色腓肠肌心磷脂和磷脂酰乙醇胺、白色腓肠肌和膈肌磷脂酰肌醇的含量。训练组红腓肠肌总磷脂含量高于对照组。训练降低了所有肌肉中鞘磷脂和心磷脂的比活性，比目鱼肌、红色和白色腓肠肌中的磷脂酰胆碱，所有肌肉中的磷脂酰丝氨酸，除比目鱼肌外的所有肌肉中的磷脂酰肌醇，后腿肌肉中的磷脂酰乙醇胺，但与久坐组中的相应值相比，膈肌中的磷脂酰乙醇胺没有降低。得出的结论是，耐力训练影响骨骼肌磷脂含量和率的血液传播的[C-14]-棕榈酸的磷脂部分的纳入。
Only few data. are available on the effect of training on phospholipid metabolism in skeletal muscles. The aim of the present study was to examine the effect of 6 weeks of endurance training on the content of particular phospholipid fractions and on the incorporation of blood-borne [C-14]-palmitic acid into the phospholipids in different skeletal muscles (white and red sections of the gastrocnemius, the soleus and the diaphragm) of the rat. Lipids were extracted from the muscles and separated using thin-layer chromatography into the following fractions: sphingomyelin, phosphatidylcholine, phosphatidylserine, phosphatidylinositol, phosphatidylethanolamine, cardiolipin and neutral lipids (this fraction being composed mostly of triacylglycerols). It was found that training did not affect the content of any phospholipid fraction in soleus muscle. It increased the content of sphingomyelin in white gastrocnemius muscle, cardiolipin and phosphatidylethanolamine in red gastrocnemius muscle and phosphatidylinositol in white gastrocnemius muscle and diaphragm. The total phospholipid content in red gastrocnemius muscle of the trained group was higher than in the control group. Training reduced the specific activity of sphingomyelin and cardiolipin in all muscles, phosphatidylcholine in soleus, red, and white gastrocnemius muscles, phosphatidylserine in all muscles, phosphatidylinositol in all except the soleus muscle, and phosphatidylethanolamine in hindleg muscles, but not in the diaphragm compared to the corresponding values in the sedentary group. It was concluded that endurance training affects skeletal muscle phospholipid content and the rate of incorporation of the blood-borne [C-14]-palmitic acid into the phospholipid moieties.