Enhancements of lipid productivity and phosphorus utilization efficiency of Chlorella pyrenoidosa by iron and acetate supplements in actual municipal wastewater
Enhancements of lipid productivity and phosphorus utilization efficiency of Chlorella pyrenoidosa by iron and acetate supplements in actual municipal wastewater
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DOI：
10.1016/j.renene.2021.01.148
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发表时间：
2021-02
期刊：
影响因子：
8.7
通讯作者：
Junzhi Liu;Jinzhou Yin;Han Houfeng-;Yaming Ge;Zheng-Yan Wang;Xingyue Bao;Feng Gao
中科院分区：
文献类型：
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作者：
Junzhi Liu;Jinzhou Yin;Han Houfeng-;Yaming Ge;Zheng-Yan Wang;Xingyue Bao;Feng Gao
营养丰富的废水被认为是生产低成本微藻生物燃料的一种很有前途的替代人工培养基质的资源。然而，要与传统的化石能源竞争，相应的藻脂生产率还需要提高。本研究通过添加铁和醋酸盐来提高蛋白核小球藻在实际生活污水中的产脂率。结果表明，同时添加适量的铁和醋酸盐可显著提高微藻细胞干重、脂肪含量和产脂率，分别提高185%、248%和671%。此外，乙酸酯的加入提高了藻基脂肪酸甲酯的饱和度(P<0.05)，从而改善了藻脂的质量，具有更好的氧化稳定性。此外，添加醋酸盐也显著提高了藻类磷的利用效率(PUE)，混合营养培养中磷向藻类DCW和脂肪的转化效率是相应光营养培养中的两倍以上。最后，报道了微藻生物生成β-磷酸三钙(β-TcP)的情况，表明微藻驱动的含磷产物的生物生产与废水除磷相结合具有潜在的磷回收潜力。
Wastewater with rich nutrients has been considered as a promising resource to replace artificial culture media in the production of low-cost microalgae biofuels. However, to compete with the traditional fossil energy, the corresponding algal lipid productivity needs to be improved. In this study, iron and acetate supplements were used to improve the lipid productivity ofChlorella pyrenoidosa in actual municipal wastewater (MW). The results showed that, simultaneously adding proper amounts of iron and acetate remarkably increased the microalga dry cell weight (DCW), lipid content and lipid productivity, respectively by 185%, 248% and 671%. Moreover, the addition of acetate increased the saturation degree of the alga based fatty acid methyl esters (P <0.05), thus improved the quality of the algal lipid with better oxidative stability. Besides, the algal phosphorus utilization efficiency (PUE) was also greatly enhanced by the acetate supplement (P <0.05), with the conversion efficiencies of phosphorus to algal DCW and lipid in mixotrophic cultures being more than twice of those in the corresponding phototrophic cultures. Finally, a bio-formation of β-tricalcium phosphate (β-TCP) by the microalga was reported, indicating potential phosphorus recovery by microalgae-driven bio-production of phosphorus-containing products combined with phosphorus removal in wastewaters.