Determination and characterization of vitamin B12 in the muscles and head innards of edible shrimp
Determination and characterization of vitamin B12 in the muscles and head innards of edible shrimp
复制标题
食用虾肌肉和头部内脏中维生素B12的测定和表征
DOI：
10.1007/s12562-019-01397-x
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发表时间：
2020
影响因子：
1.9
通讯作者：
F. Watanabe
中科院分区：
文献类型：
--
作者：
Naho Okamoto;Natsumi Hamaguchi;Y. Umebayashi;S. Takenaka;T. Bito;F. Watanabe
我们用基于德氏乳杆菌的微生物测定法测定了不同种类对虾[Argis larl(Owen，1839)；Togezako虾，Argis toyamaensis(Yokoya，1933)；Pandalopsis Japan，Balss，1914；Pandalus eousMakarov，1935]肌肉和头部中维生素B12的含量。在对虾肌肉中检测到大约2-4微克维生素B12/100微克湿重-相当大的量-。对虾头部内脏含有明显较高水平的维生素B12(~ 12-33微克/100微克湿重)。市面上出售的虾内脏产品含有 ~ 30微克维生素B12/100微克湿重。我们用免疫亲和层析柱从对虾肌肉和头部内脏提取液中纯化维生素B12化合物。肌肉提取物中只含有1种皮质醇化合物，经高效液相色谱-电喷雾电离/串联质谱仪鉴定为维生素B12，而虾头内脏含有3种皮质醇化合物，其中含有大量的维生素B12和两种少量的维生素B12-d-单羧酸，初步鉴定为维生素B12二元酸。
We determined the vitamin B12content in both the muscles and head innards of various shrimp species [Argis lar(Owen, 1839); Togezako shrimp,Argis toyamaensis(Yokoya, 1933);Pandalopsis japonica, Balss, 1914;Pandalus eousMakarov, 1935] using a microbiological assay based onLactobacillus delbrueckiisubsp.lactisATCC7830. Approximately 2–4 µg vitamin B12/100 g wet weight—a considerable amount—was detected in shrimp muscles. The shrimp head innards contained significantly higher levels of vitamin B12(~ 12–33 µg/100 g wet weight). Commercially available shrimp-innard products contained ~ 30 µg vitamin B12/100 g wet weight. We purified vitamin B12compounds from the extracts of shrimp muscles and head innards using an immunoaffinity column. The muscle extract contained only one corrinoid compound, which was identified as vitamin B12using liquid chromatography–electrospray ionization/tandem mass spectrometry, whereas the shrimp head innards contained three corrinoid compounds, which included large amounts of vitamin B12and two smaller amounts of vitamin B12-d-monocarboxylic acid and tentatively identified vitamin B12dicarboxylic acids.