Immunization of male but not female mice with the sperm-specific isozyme of lactate dehydrogenase (LDH-C4) impairs fertilization in vivo.
Immunization of male but not female mice with the sperm-specific isozyme of lactate dehydrogenase (LDH-C4) impairs fertilization in vivo.
复制标题
用精子特异性乳酸脱氢酶（LDH-C4）同工酶对雄性小鼠（而不是雌性小鼠）进行免疫会损害体内受精。
DOI：
10.1111/j.1600-0897.1990.tb00687.x
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Goldberg,E
中科院分区：
文献类型：
--
作者：
Mahi-Brown,CA;VandeVoort,CA;McGuinness,RP;Overstreet,JW;O'Hern,P;Goldberg,E
The goals of this study were to determine the site at which fertility is impaired in mice immunized with LDH‐C4and to determine whether immunization of both males and females would have a greater antifertility effect than immunization of one sex alone. Mice were immunized with LDH‐C4in two systemic doses in Freund's adjuvants and two gastric doses in bicarbonate buffer. The presence of anti‐LDH‐C4antibodies in uterine fluid was confirmed. Male and female mice were assigned to four blocks in which either the males, the females, both, or neither were immunized. Oviducts were viewed directly 1 h after mating for the presence of sperm. No significant effect of immunization on sperm transport to the oviduct could be demonstrated. Fertilization was evaluated 4 h after mating. It was found that immunization of males, but not females, impaired fertilization (19.6% versus 50.8% of the oocytes penetrated in 17 and 19 females, respectively). Orchitis was found histologically in 43.8% of the immunized males and 10% of the control males.
