Cholinesterase activity and choline uptake in intact nerve cell cultures
Cholinesterase activity and choline uptake in intact nerve cell cultures
复制标题
完整神经细胞培养物中的胆碱酯酶活性和胆碱摄取
DOI：
10.1016/0006-8993(76)90337-1
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发表时间：
1976
期刊：
影响因子：
2.9
通讯作者：
P. Mandel
中科院分区：
文献类型：
--
作者：
R. Massarelli;V. Stefanović;P. Mandel
在完整的星形细胞和神经母细胞培养中，已经测量了胆碱摄取和外胆碱酯酶活性。这些数据表明，胆碱的摄取依赖于培养基离子组成，并且对代谢抑制剂敏感。然而，抑制吸收所需的高浓度抑制剂和某些热力学性质表明，这是一个促进运输的过程，而不是一个活跃的上坡过程。用不同的胆碱酯酶抑制剂预先孵育培养细胞，发现抑制高亲和力胆碱摄取(明显是Km≊10−6M)和抑制外乙酰胆碱酯酶(EC3.1.1.7)之间没有直接平行关系。
Choline uptake and ecto-cholinesterase activities have been measured in intact astroblast and neuroblast cultures. The data show that choline uptake is dependent upon the ionic composition of the culture medium and is sensitive to metabolic inhibitors. However, the high concentrations of the inhibitors necessary for the inhibition of the uptake and some thermodynamic properties would suggest a facilitated transport rather than an active uphill process. Preincubation of the cultures with various inhibitors of cholinesterases shows no direct parallelism between inhibition of choline high affinity uptake (apparentKm≊ 10−6M) and inhibition of ecto-acetylcholinesterase (EC 3.1.1.7).