Correlation between local rriionocarboxylate transporter 1 (MCTI) and glucose transporter 1 (GLUTI) densities in the adult rat brain
Correlation between local rriionocarboxylate transporter 1 (MCTI) and glucose transporter 1 (GLUTI) densities in the adult rat brain
复制标题
DOI：
10.1016/j.neulet.2003.10.056
复制
发表时间：
2004-01-23
影响因子：
2.5
通讯作者：
Duelli, R
中科院分区：
文献类型：
--
作者：
Maurer, MH;Canis, M;Duelli, R
单羧酸转运蛋白1型（MCT 1）促进单羧酸跨血脑屏障和脑实质细胞的细胞膜转运。本研究有两个目的：（1）确定MCT 1在脑中的局部分布;（2）比较MCT 1的局部密度与主要营养转运蛋白葡萄糖转运蛋白GLUT 1的局部密度。用免疫放射自显影法对大鼠脑冷冻切片进行了分析，共分析了32个脑结构。(1)在整个脑中观察到MCT 1密度的异质性分布模式。与脑匀浆（100%）相比，研究的脑结构中MCT 1密度范围为43%至164%。局部GLUT 1密度显示出相当的范围（35-145%）。(2)发现局部MCT 1和局部GLUT 1密度之间存在密切相关性。由于局部GLUT 1密度反映了大脑中的局部葡萄糖代谢，因此我们得出结论，局部MCT 1密度根据局部葡萄糖代谢和转运进行调整。(C)2003爱思唯尔爱尔兰有限公司保留所有权利。
Monocarboxylate transporters type 1 (MCT1) facilitate the transport of monocarboxylates across cell membranes of the blood-brain barrier and brain parenchymal cells. The present study had two aims: (1) to determine the local distribution of MCT1 in the brain; and (2) to compare the local densities of MCT1 with the local densities of the main nutritional transporters, glucose transporter GLUT1. Using immunoautoradiography of cryosections from rat brain,32 brain structures were analyzed. (1) A heterogenous distribution pattern of MCT1 densities was observed throughout the brain. Compared to brain homogenate (100%), MCT1 densities ranged from 43 to 164% in the brain structures investigated. Local GLUT1 densities showed a comparable range (35-145%). (2) A close correlation was found between local MCT1 and local GLUT1 densities. As local GLUT1 densities reflect local glucose metabolism in the brain, we conclude that local MCT1 densities are adjusted to local glucose metabolism and transport. (C) 2003 Elsevier Ireland ltd. All rights reserved.