Analysis of vitamin D-dependent calcium-binding protein messenger ribonucleic acid expression in mice lacking the vitamin D receptor
Analysis of vitamin D-dependent calcium-binding protein messenger ribonucleic acid expression in mice lacking the vitamin D receptor
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DOI：
10.1210/en.139.3.847
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发表时间：
1998-03-01
期刊：
影响因子：
4.8
通讯作者：
Demay, MB
中科院分区：
文献类型：
--
作者：
Li, YC;Pirro, AE;Demay, MB
To investigate the roles of the receptor-dependent actions of 1,25-dihydroxyvitamin D-3 [1,25-(OH)(2)D-3] in the regulation of vitamin D-dependent calcium-binding proteins (calbindin-D), the messenger RNA (mRNA) levels of calbindin-D9k and -28k were examined in vitamin D receptor (VDR)-ablated mice and control littermates. In VDR-ablated mice, calbindin-D9k mRNA was dramatically reduced in the intestine, kidneys, lungs, and brain; however, calbindin-D28k mRNA was only moderately decreased in the kidney. After 1,25-(OH)(2)D-3 injection, calbindin-D9k mRNA levels and renal and alveolar calbindin-D28k mRNA levels were induced in control animals, but not in the homozygous mice.When the mice were fed a diet high in lactose, calcium, and phosphorus, intestinal calbindin-D9k mRNA levels in the homozygous mice were restored to those in their control littermates. However, this diet failed to normalize extraintestinal calbindin mRNA levels.These findings demonstrate that the receptor-dependent actions of 1,25-(OH)(2)D-3 regulate calbindin-D9k gene expression and that tissue-specific factors modulate the effects of 1,25-(OH),D, on calbindin-D28k gene expression. These data also demonstrate that in the absence of a functional VDR, a high local concentration of calcium, phosphorus, and/or lactose in the intestinal lumen can normalize intestinal calbindin-D9k, mRNA levels.
