BRLA IS NECESSARY AND SUFFICIENT TO DIRECT CONIDIOPHORE DEVELOPMENT IN ASPERGILLUS-NIDULANS
BRLA IS NECESSARY AND SUFFICIENT TO DIRECT CONIDIOPHORE DEVELOPMENT IN ASPERGILLUS-NIDULANS
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DOI：
10.1016/0092-8674(88)90198-5
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发表时间：
1988-07-29
期刊：
影响因子：
64.5
通讯作者：
TIMBERLAKE, WE
中科院分区：
文献类型：
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作者：
ADAMS, TH;BOYLAN, MT;TIMBERLAKE, WE
将A. nidulans介导从营养细胞的不确定的顶端生长模式到分生孢子梗的出芽生长模式的发育转换。brlA编码432个氨基酸的多肽，其含有两个直接重复的基序，类似于首先在非洲爪蟾TFIIIA中识别的Zn（II）配位位点。brlA在营养细胞中的误表达导致发育调控基因的转录激活、单向菌丝生长的停止、类似于在正常分生孢子柄发育期间发生的细胞转化的启动以及有活力的分生孢子的产生。我们认为BRLA是一种核酸结合蛋白，其在营养细胞中的表达足以通过其调节分生孢子特异性基因表达的作用诱导孢子形成。
The brlA gene of a A. nidulans mediates the developmental switch from indeterminate, apical growth pattern of vegetative cells to the budding growth pattern of conidiophores. brlA encodes a 432 amino acid polypeptide containing two directly repeated motifs resembling the Zn(II) coordination sites first recognized in Xenopus TFIIIA. Misscheduled expression of brlA in vegetative cells results in transcriptional activation of developmentally regulated genes, cessation of unidirectional hyphal growth, initiation of cellular transformations resembling those that occur during normal conidiophore development, and production of viable conidiospores. We propose that BRLA is a nucleic acid-binding protein whose expression in vegetative cells is sufficient to induce sporulation through its role in regulating expression of conidiation-specific genes.