Detection of bovine tuberculosis in herds with different disease prevalence and influence of paratuberculosis infection on PPDB and ESAT-6/CFP10 specificity
Detection of bovine tuberculosis in herds with different disease prevalence and influence of paratuberculosis infection on PPDB and ESAT-6/CFP10 specificity
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DOI：
10.1016/j.prevetmed.2010.06.007
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发表时间：
2010-09-01
影响因子：
2.6
通讯作者：
Cataldi, A.
中科院分区：
文献类型：
--
作者：
Aagaard, C.;Govaerts, M.;Cataldi, A.
牛结核病（BTB）是一种具有人畜共患病影响的主要动物健康问题。目前的控制计划基于使用结核菌素作为抗原的皮肤测试的测试和屠宰策略。这些测试的低特异性和相关的操作困难推动了对新抗原和诊断测定的寻找。在这项多中心研究中，我们使用来自阿根廷、墨西哥和北爱尔兰的牛群，从 BTB 患病率不同的牛群中选择皮试阳性和阴性动物，并比较结核菌素 (PPDB) 和 ESAT-6 + CFP10 作为离体抗原。在低流行率牛群中，PPDB 的交叉反应性很明显，因为高达 60% 的 PPDB 皮试和离体阳性动物对 ESAT-6 + CFP10 离体没有反应。 ESAT-6 + CFP10 的卓越特异性在鸟分枝杆菌中得到证实。副结核病感染的牛群，其中一些动物对 PPDB 和 PPDA 有很强的交叉反应性，但对 ESAT-6 + CFP10 没有。在高患病率的畜群中，85% 的皮肤测试呈阳性的动物是使用 PPDB 或 ESAT-6 + CFP10 作为抗原离体确认的。然而，在该组中，60% 的皮试阴性动物在体外呈 PPDB 和 ESAT-6 + CFP10 阳性，这表明皮试可能在某些畜群中产生大量假阴性结果。总之，建议将体外试验作为加速 BTB 根除的辅助试验。在高流行率牛群中，可以使用 PPDB 或 ESAT-6 + CFP10 作为抗原，而在中低流行率牛群中，ESAT-6 + CFP10 是首选。 (C) 2010 Elsevier B.V. 保留所有权利。
Bovine tuberculosis (BTB) is a major animal health problem with zoonotic implications. Current control programs are based on test and slaughter strategies utilizing skin tests with tuberculins as antigens. The low specificity and associated operative difficulties of these tests have driven the search for new antigens and diagnostic assays. In this multi-center study, using herds from Argentina, Mexico and Northern Ireland, we selected skin test positive and negative animals from herds with different prevalence's of BTB and compared tuberculin (PPDB) and ESAT-6 + CFP10 as antigens ex vivo. In low prevalence herds, crossreactivity of PPDB was apparent since up to 60% of the PPDB skin test and ex vivo positive animals did not responded to ESAT-6 + CFP10 ex vivo. The superior specificity of ESAT-6 + CFP10 was confirmed in a Mycobacterium avium sp. paratuberculosis infected herd where several of the animals had strong crossreactivity to PPDB and PPDA but not to ESAT-6 + CFP10. In high prevalence herds 85% of the skin test-positive animals, were confirmed ex vivo using either PPDB or ESAT-6 + CFP10 as antigen. However, within this group 60% of the skin test negative animals were PPDB and ESAT-6 + CFP10 positive ex vivo indicating that the skin test can in some herds yield a significant number of false negative results. In conclusion, the ex vivo test is recommended as an ancillary test to accelerate BTB eradication. In high prevalence herds, PPDB or ESAT-6 + CFP10 can be used as antigen whereas in low and medium prevalence herds ESAT-6 + CFP10 is the preferred choice. (C) 2010 Elsevier B.V. All rights reserved.