Regional fractional ventilation mapping in spontaneously breathing mice using hyperpolarized 129Xe MRI
Regional fractional ventilation mapping in spontaneously breathing mice using hyperpolarized 129Xe MRI
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DOI：
10.1002/nbm.3222
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发表时间：
2015-01-01
影响因子：
2.9
通讯作者：
Kimura, Atsuomi
中科院分区：
文献类型：
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作者：
Imai, Hirohiko;Matsumoto, Hironobu;Kimura, Atsuomi
超极化 (HP) Xe-129 MRI 通气成像的可行性已被研究用于对自主呼吸小鼠的通气进行定量和区域评估。多次呼吸通气成像技术被修改为从 Xe-129 偏振器连续输送 HP Xe-129 的自发吸入方案。通过在 Xe-129 肺部成像之前改变呼吸次数获得一系列 Xe-129 通气图像。成功评估了自主呼吸小鼠的通气分数 r。尝试检测气管内施用猪胰弹性蛋白酶（PPE）引起的肺气肿小鼠肺通气功能障碍。因此，通气分数的分布可以通过 r 图可视化。在 PPE 治疗组中定量发现了显着的通气功能障碍。对照小鼠 (N=4) 的全肺 r 值为 0.34 +/- 0.01，PPE 处理的小鼠 (N=4) 显着降低至 0.25 +/- 0.07 (p=0.038)。这项研究是首次将多次呼吸通气成像应用于自主呼吸的小鼠，并表明该方法对肺通气的差异敏感。在评估小型啮齿动物的区域通气时，该方法有望提高简单性和无创性。版权所有 (c) 2014 约翰·威利父子有限公司
The feasibility of ventilation imaging with hyperpolarized (HP) Xe-129 MRI has been investigated for quantitative and regional assessment of ventilation in spontaneously breathing mice. The multiple breath ventilation imaging technique was modified to the protocol of spontaneous inhalation of HP Xe-129 delivered continuously from a Xe-129 polarizer. A series of Xe-129 ventilation images was obtained by varying the number of breaths before the Xe-129 lung imaging. The fractional ventilation, r, was successfully evaluated for spontaneously breathing mice. An attempt was made to detect ventilation dysfunction in the emphysematous mouse lung induced by intratracheal administration of porcine pancreatic elastase (PPE). As a result, the distribution of fractional ventilation could be visualized by the r map. Significant dysfunction of ventilation was quantitatively identified in the PPE-treated group. The whole-lung r value of 0.34 +/- 0.01 for control mice (N=4) was significantly reduced, to 0.25 +/- 0.07, in PPE-treated mice (N=4) (p=0.038). This study is the first application of multiple breath ventilation imaging to spontaneously breathing mice, and shows that this methodology is sensitive to differences in the pulmonary ventilation. This methodology is expected to improve simplicity as well as noninvasiveness when assessing regional ventilation in small rodents. Copyright (c) 2014 John Wiley & Sons, Ltd.