Phenotypic changes of human neuroblastoma cells in culture induced by 12‐O‐tetradecanoyl‐phorbol‐13‐acetate
Phenotypic changes of human neuroblastoma cells in culture induced by 12‐O‐tetradecanoyl‐phorbol‐13‐acetate
复制标题
12-O-十四烷酰佛波醇-13-乙酸酯诱导培养物中人神经母细胞瘤细胞的表型变化
DOI：
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发表时间：
1981
影响因子：
6.4
通讯作者：
K. Nilsson
中科院分区：
文献类型：
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作者：
S. Påhlman;L. Odelstad;E. Larsson;G. Grotte;K. Nilsson
SK‐N‐SH and SH‐SYSY human neuroblastoma cells treated by 12‐O‐tetradecanoyl‐phorbol‐13‐acetate (TPA) express morphological and biochemical changes, which indicate that differentiation towards more mature cells has occurred. The most prominent morphological changes were the development in 40–60% of the cells of cell‐surface projections longer than 50 μm and cytoplasmic neurosecretory granules demonstrated by electron microscopy. At the biochemical level, TPA induced a two‐fold increase in the relative activity of neuron‐specific enolase and 30‐ to 40‐fold increase in noradrenaline and adrenaline concentrations. A decrease in proliferation rate of TPA‐treated cells was observed. The biological effects of TPA were slightly potentiated by nerve growth factor.
