Whole Genome Sequencing Reveals the Structure of Environment-Associated Divergence in a Broadly Distributed Montane Bumble Bee, Bombus vancouverensis
Whole Genome Sequencing Reveals the Structure of Environment-Associated Divergence in a Broadly Distributed Montane Bumble Bee, Bombus vancouverensis
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DOI：
10.1093/isd/ixac025
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发表时间：
2022-09-01
影响因子：
3.4
通讯作者：
Ware, Jessica
中科院分区：
文献类型：
--
作者：
Heraghty, Sam D.;Rahman, Sarthok Rasique;Ware, Jessica
分布广泛的物种在其分布范围内经历着不同的非生物条件，这可能会推动当地的适应。由于温度、氧气供应和空气密度等多种非生物因素的空间差异，种群分布在纬度和海拔梯度上的山地系统特别可能产生局部适应。我们利用全基因组测序技术对分布于北美西部的温哥华熊蜂(膜翅目：蜂科)景观基因组学进行了研究。综合统计方法揭示了该地区具有异常分化程度的几个大的离群值SNPs窗口，并表明等温性和海拔高度是与这些变异最密切相关的环境特征。在这些区域内发现的基因具有不同的生物学功能，但包括神经肌肉功能、离子稳态、氧化应激和低氧，这些基因可能与对温度、干燥或高海拔条件的耐受有关。全基因组测序方法显示，在连锁不平衡升高、平均F-ST、F-升高和群体内核苷酸多样性低的基因组区域出现异常值。其他类型的结构变化与环境预测因素没有广泛的联系，但与地理分离大体一致。结果与其他研究一致，表明低重组区域可能在熊蜂物种内和物种之间存在适应性变异，并加深了我们对候选基因的理解，这些候选基因可以作为温哥华熊蜂范围内可能的选择目标进行进一步研究。
Broadly distributed species experience divergent abiotic conditions across their ranges that may drive local adaptation. Montane systems where populations are distributed across both latitudinal and elevational gradients are especially likely to produce local adaptation due to spatial variation in multiple abiotic factors, including temperature, oxygen availability, and air density. We use whole-genome resequencing to evaluate the landscape genomics of Bombus vancouverensis Cresson (Hymenoptera: Apidae), a common montane bumble bee that is distributed throughout the western part of North America. Combined statistical approaches revealed several large windows of outlier SNPs with unusual levels of differentiation across the region and indicated that isothermality and elevation were the environmental features most strongly associated with these variants. Genes found within these regions had diverse biological functions, but included neuromuscular function, ion homeostasis, oxidative stress, and hypoxia that could be associated with tolerance of temperature, desiccation, or high elevation conditions. The whole-genome sequencing approach revealed outliers occurred in genome regions with elevated linkage disequilibrium, elevated mean F-ST,F- and low intrapopulation nucleotide diversity. Other kinds of structural variations were not widely associated with environmental predictors but did broadly match geographic separation. Results are consistent with other studies suggesting that regions of low recombination may harbor adaptive variation in bumble bees within as well as between species and refine our understanding of candidate genes that could be further investigated as possible targets of selection across the B. vancouverensis range.