Mouse angiotensin-converting enzyme is a protein composed of two homologous domains.
Mouse angiotensin-converting enzyme is a protein composed of two homologous domains.
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DOI：
10.1016/s0021-9258(18)80158-2
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发表时间：
1989-07
期刊：
影响因子：
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通讯作者：
Kenneth E. BernsteinS;Brian M. Martins;A. S. Edwards;E. Bernstein
中科院分区：
文献类型：
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作者：
Kenneth E. BernsteinS;Brian M. Martins;A. S. Edwards;E. Bernstein
血管紧张素转换酶（ACE）是一种二肽羧肽酶，可将血管紧张素I转化为有效的血管收缩剂血管紧张素II。我们已经使用cDNA和基因组序列组装的复合cDNA，ACE。315，编码小鼠转化酶的整个氨基酸序列。ACE.315含有4838个碱基对，去除34个氨基酸的信号肽后，编码1278个氨基酸（147.4 kDa）的蛋白质。在蛋白质中，有两个大的同源序列区域，每个区域都含有潜在的Zn结合区域和催化位点。这些同源区域大约是整个ACE蛋白的一半大小，表明现代ACE基因是前体基因的复制产物。小鼠ACE在核酸和氨基酸序列上与人ACE 83%同源，并且与人ACE一样，在羧基末端含有疏水区，其可能将酶锚定到细胞膜上（Soubrier，F.，Alhenc-Gelas，F.，休伯特，C.，J.阿莱格里尼约翰，M.，Tregear，G.，和Corvol，P.（1988）Proc.Natl. Acad. Sci. U.S.A.85，9386-9390）。对小鼠肾脏、肺和睾丸RNA的北方分析表明，睾丸ACE同工酶由一个单一的RNA（2500个碱基）编码，比肾脏或肺中发现的两个信息大小（4900和4150个碱基）小，并且该睾丸RNA与ACE的3′部分杂交。
Angiotensin-converting enzyme (ACE) is a dipeptidyl carboxypeptidase that converts angiotensin I into the potent vasoconstrictor angiotensin II. We have used cDNA and genomic sequences to assemble a composite cDNA, ACE.315, encoding the entire amino acid sequence of mouse converting enzyme. ACE.315 contains 4838 base pairs and encodes a protein of 1278 amino acids (147.4 kDa) after removal of a 34-amino acid signal peptide. Within the protein, there are two large areas of homologous sequence, each containing a potential Zn-binding region and catalytic site. These homologous regions are approximately half the size of the whole ACE protein and suggest that the modern ACE gene is the duplicated product of a precursor gene. Mouse ACE is 83% homologous to human ACE in both nucleic acid and amino acid sequence, and like human ACE, contains a hydrophobic region in the carboxyl terminus that probably anchors the enzyme to the cell membrane (Soubrier, F., Alhenc-Gelas, F., Hubert, C., Allegrini, J., John, M., Tregear, G., and Corvol, P. (1988)Proc. Natl. Acad. Sci. U.S.A.85, 9386–9390). Northern analysis of mouse kidney, lung, and testis RNA demonstrates that the testicular isozyme of ACE is encoded by a single, smaller RNA (2500 bases) than the two message sizes found in kidney or lung (4900 and 4150 bases), and that this testicular RNA hybridizes to the 3′ portion of ACE.315.