Cross-pathway regulation in Saccharomyces cerevisiae: activation of the proline utilization pathway by Ga14p in vivo.
Cross-pathway regulation in Saccharomyces cerevisiae: activation of the proline utilization pathway by Ga14p in vivo.
复制标题
酿酒酵母中的跨途径调节：体内 Ga14p 激活脯氨酸利用途径。
DOI：
10.1128/jb.182.13.3748-3753.2000
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发表时间：
2000
影响因子：
3.2
通讯作者：
Brandriss,MC
中科院分区：
文献类型：
--
作者：
D'Alessio,M;Brandriss,MC
Put3p和Gal4p转录激活因子是一类不同的真菌调节因子，称为Cys6Zn（II）2双核簇家族。这个家族包括超过50种不同的酵母菌蛋白，它们共享相似的结构域组织。Gal4p激活半乳糖利用途径的基因，允许使用半乳糖作为碳和能量的唯一来源。Put3p控制脯氨酸利用途径的表达，该途径允许酵母细胞在脯氨酸作为唯一氮源上生长。我们报道Gal4p可以激活Put 3p缺失菌株中的Put结构基因。我们还表明，激活ofPUT2的Gal4p依赖于诱导剂半乳糖和Put3p结合位点的存在下，激活增加Gal4p的剂量。Put3p在缺乏Gal4p的情况下不能激活GAL基因。我们的体内结果证实了先前发表的体外数据，表明Gal4p在其DNA结合能力方面比Put3p更混杂。结果还表明，在适当的情况下，Gal4p可能能够代替相关家族成员激活表达。
The Put3p and Gal4p transcriptional activators are members of a distinct class of fungal regulators called the Cys6Zn(II)2binuclear cluster family. This family includes over 50 differentSaccharomyces cerevisiaeproteins that share a similar domain organization. Gal4p activates the genes of the galactose utilization pathway permitting the use of galactose as the sole source of carbon and energy. Put3p controls the expression of the proline utilization pathway that allows yeast cells to grow on proline as the sole nitrogen source. We report that Gal4p can activate thePUTstructural genes in a strain lacking Put3p. We also show that the activation ofPUT2by Gal4p depends on the presence of the inducer galactose and the Put3p binding site and that activation increases with increased dosage of Gal4p. Put3p cannot activate theGALgenes in the absence of Gal4p. Our in vivo results confirm previously published in vitro data showing that Gal4p is more promiscuous than Put3p in its DNA binding ability. The results also suggest that under appropriate circumstances, Gal4p may be able to function in place of a related family member to activate expression.