Purification, characterization, and cDNA cloning of a novel growth factor from the conditioned medium of NIH-Sape-4, an embryonic cell line of Sarcophaga peregrina (flesh fly)
Purification, characterization, and cDNA cloning of a novel growth factor from the conditioned medium of NIH-Sape-4, an embryonic cell line of Sarcophaga peregrina (flesh fly)
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DOI：
10.1074/jbc.271.23.13770
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发表时间：
1996-06-07
影响因子：
4.8
通讯作者：
Natori, S
中科院分区：
文献类型：
--
作者：
Homma, K;Matsushita, T;Natori, S
将来自肉蝇胚胎细胞系 NIH-Sape-4 条件培养基的生长因子纯化至均质。这种生长因子被称为 IDGF，以自分泌方式刺激 NIH-Sape-4 细胞的增殖。它是一种分子量为52 kDa的蛋白质的同型二聚体，其比活性与哺乳动物生长因子相当。免疫印迹实验表明，肉蝇未受精的成熟卵中含有该生长因子，并在整个胚胎发育过程中保持一定水平。对该生长因子的cDNA分析表明，该因子是一种由553个氨基酸残基组成的新型蛋白质。该因子与除心房腺颗粒特异性抗原外的其他蛋白质没有发现显着的序列相似性。加州海兔。
A growth factor from the conditioned medium of NIH-Sape-4, an embryonic cell line of the flesh fly, was purified to homogeneity. This growth factor, termed IDGF, stimulated the proliferation of NIH-Sape-4 cells in an autocrine manner; it was a homodimer of a protein with a molecular mass of 52 kDa, and its specific activity was comparable with those of mammalian growth factors, Immunoblotting experiments revealed that unfertilized mature eggs of the flesh fly contained this growth factor, a certain level of which was maintained throughout embryonic development, Analysis of cDNA for this growth factor showed that this factor is a novel protein consisting of 553 amino acid residues, No significant sequence similarity was found between this factor and other proteins except atrial gland granule-specific antigen of Aplysia californica.