Target-dependent hormonal control of neuron size in the rat spinal nucleus of the bulbocavernosus
Target-dependent hormonal control of neuron size in the rat spinal nucleus of the bulbocavernosus
复制标题
大鼠球海绵体脊髓核神经元大小的目标依赖性激素控制
DOI：
10.1523/jneurosci.11-10-03025.1991
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发表时间：
1991
期刊：
影响因子：
2.9
通讯作者：
M. Kuno
中科院分区：
文献类型：
--
作者：
I. Araki;Y. Harada;M. Kuno
大鼠球海绵体肌脊核（SNB）是一组支配会阴肌的两性异形运动神经元。在成年雄性大鼠中，SNB神经元的大小在去势后减少，并且这种作用通过睾酮治疗逆转。然而，雄激素受体存在于会阴肌肉以及SNB神经元中。因此，目前尚不清楚神经元的大小是由激素对SNB神经元的直接作用调节，还是由靶源性因子调节，靶源性因子可通过激素对神经支配肌肉的作用来控制。为了解决这个问题，切断一侧SNB神经元的外周（阴部）神经，并将其与缺乏雄激素敏感性的移植比目鱼肌联合。在对照侧，阴部神经被类似地切断，但允许会阴肌肉再神经支配。SNB神经元的大小在术后10周后测量两侧，在此期间动物已被阉割或阉割后用睾酮处理。SNB神经元的大小，已重新支配会阴肌肉减少去势后，扩大睾酮治疗。相比之下，SNB神经元的大小，支配比目鱼肌保持不变，响应睾酮操纵。它的结论是激素调节成年大鼠SNB神经元的大小是由它们的靶肌肉介导的。
The spinal nucleus of the bulbocavernosus (SNB) in the rat is a cluster of sexually dimorphic motoneurons that innervate perineal muscles. In adult male rats, the size of SNB neurons is reduced following castration, and this effect is reversed by treatment with testosterone. However, androgen receptors are present in the perineal muscles as well as in SNB neurons. Therefore, it is not clear whether the neuronal size is regulated by direct action of the hormone on SNB neurons or by a target-derived factor that may be controlled via hormonal action on the innervated muscle. To address this question, the peripheral (pudendal) nerve of SNB neurons on one side was cut and united to the grafted soleus muscle, which lacks androgen sensitivity. On the control side, the pudendal nerve was similarly cut but was allowed to reinnervate the perineal muscles. The size of SNB neurons was measured on both sides after a postoperative period of 10 weeks, during which the animal had been castrated or treated with testosterone after castration. The size of SNB neurons that had reinnervated perineal muscles was reduced following castration and enlarged by testosterone treatment. In contrast, the size of SNB neurons that had innervated the soleus muscle remained unaltered in response to testosterone manipulation. It is concluded that hormonal regulation of the size of SNB neurons in adult rats is mediated by their target muscles.