Feed-forward regulation of gibberellin deactivation in pea
Feed-forward regulation of gibberellin deactivation in pea
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DOI：
10.1007/s003440010004
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发表时间：
2001-03-01
影响因子：
4.8
通讯作者：
Reid, JB
中科院分区：
文献类型：
--
作者：
Elliott, RC;Ross, JJ;Reid, JB
多种证据表明，参与赤霉素（GA）生物合成的基因受到生物活性GA水平的调节。最近克隆的GA 2-氧化酶基因从豌豆，我们调查是否这种稳态调节延伸到控制GA失活的基因在这个物种中，利用两个良好的特点GA缺陷突变体，LS和NA和GA积累突变体，SLN。豌豆GA 2-氧化酶在mRNA水平上表现出前馈效应，而内源GA（20）、GA（29）、GA（1）和GA（8）水平上没有表现出前馈调节的证据。基因组Southern印迹和表达序列标签（EST）数据库的分析表明，其他GA 2-氧化酶可能占这种缺乏前馈GA水平。
Multiple lines of evidence suggest that the genes involved in gibberellin (GA biosynthesis are regulated by bioactive GA levels. With the recent cloning of GA 2-oxidase genes from pea, we investigated whether this homeostatic regulation extends to the genes controlling GA deactivation in this species, utilizing two well-characterized GA-deficient mutants, ls and na and a GA-accumulating mutant, sln. The pea GA 2-oxidases showed feed-forward effects at the mRNA level, while the endogenous levels of GA(20), GA(29), GA(1), and GA(8) showed no evidence of feed-forward regulation. Analyses of genomic Southern blots and expressed sequenced tag (EST) databases suggest that other GA 2-oxidases could possibly account for this lack of feed-forward on GA levels.