Effects of repeated nerve injuries at different time intervals on functional recovery and nerve innervation
Effects of repeated nerve injuries at different time intervals on functional recovery and nerve innervation
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不同时间间隔重复神经损伤对功能恢复和神经支配的影响
DOI：
10.1016/j.jocn.2017.11.016
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发表时间：
2018
影响因子：
2
通讯作者：
N. Fukushima
中科院分区：
文献类型：
--
作者：
M. Karasawa;K. Yokouchi;K. Kawagishi;T. Moriizumi;N. Fukushima
研究了反复神经损伤对啮齿类动物功能恢复和神经支配的影响。对成年大鼠造成坐骨神经挤压伤，每隔1、2、3、4 周重复2~3次。在最后一次挤压后1、7、14、21、28、35、42、49和56 用静态坐骨神经指数评估运动功能。用βⅢ型微管蛋白阳性神经末梢和α银环蛇毒素阳性乙酰胆碱受体定量测定三重神经损伤后21、56 d、1周、2周、3周和4周时腓总神经支配的主要肌肉胫前肌的神经支配比率。与单纯神经挤压伤相比，反复挤压伤运动功能恢复延迟。此外，三次挤压组的恢复速度慢于二次挤压组。除21 后1周组(73.8%)和2周组(70.5%)外，末次挤压后21 d(单次挤压：59.7%；三次挤压：54.1%~56.1%)和56 d(单一挤压：88.8%；三次挤压：72.5%~83.0%)的神经再发率均低于一次挤压组。最后一次挤压伤后8 ，三次挤压组大鼠运动功能恢复延迟，神经支配发生率仍较低。
Effects of repeated nerve injuries on functional recovery and nerve innervation were examined in rodents. Crush injuries of the sciatic nerve were inflicted on adult rats and repeated twice or thrice at different time intervals of 1, 2, 3, and 4 weeks. Motor function was assessed by the static sciatic index at 1, 7, 14, 21, 28, 35, 42, 49, and 56 days after the final crush. The rates of nerve innervation of the tibialis anterior muscle, a main muscle innervated by the common peroneal nerve, were evaluated by the quantification of βIII-tubulin-positive nerve terminals and α-bungarotoxin-positive acetylcholine receptors 21 and 56 days after the final crush of triple nerve injuries at 1-, 2-, 3-, and 4-week intervals. Compared with single nerve crush injury, delayed recovery of motor function was observed in repeated crush injuries. In addition, recoveries in the triple crush groups were slower than those in the double crush groups. The rates of reinnervation were lower in the triple crush groups than in the single crush groups, both at 21 days (single: 59.7%; triple: 54.1%–56.1%) and 56 days (single: 88.8%; triple: 72.5%–83.0%) after the final crush, except in the groups with 1-week (triple: 73.8%) and 2-week (triple: 70.5%) intervals at 21 days after the final crush. We concluded that the recovery of motor function was delayed according to the number of repetitions of crush injuries, and that the rates of nerve innervation were still low in the triple crush groups 8 weeks after the final crush.