POSTNATAL-DEVELOPMENT OF THE SERTOLI-CELL BARRIER, TUBULAR LUMEN, AND CYTOSKELETON OF SERTOLI AND MYOID CELLS IN THE RAT, AND THEIR RELATIONSHIP TO TUBULAR FLUID SECRETION AND FLOW
POSTNATAL-DEVELOPMENT OF THE SERTOLI-CELL BARRIER, TUBULAR LUMEN, AND CYTOSKELETON OF SERTOLI AND MYOID CELLS IN THE RAT, AND THEIR RELATIONSHIP TO TUBULAR FLUID SECRETION AND FLOW
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DOI：
10.1002/aja.1001840302
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发表时间：
1989-03-01
影响因子：
--
通讯作者：
GOH, JC
中科院分区：
文献类型：
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作者：
RUSSELL, LD;BARTKE, A;GOH, JC
在Sprague-Dawley大鼠中研究了支持细胞屏障、管腔、流体流动和支持细胞和肌样细胞中的细胞骨架元素的出生后发育。在高渗固定剂的帮助下，在出生后第15和16天（p.n.），在大多数小管中观察到液体快速进入的障碍。d，在p.n.前在所有小管中完全形成。第18天。形成外质特化（ES）的肌动蛋白是一种与构成屏障的封闭连接相关的细胞骨架复合物，在最初的屏障形成期间（16 p.n.日，以其成熟而为之。在p.n.左右，ES形成了成虫特征性的荧光模式和荧光强度。第二十二天。早在p.n.，一些曲细精管显示出非常小的管腔。第10天所有小管在p.n.前均未打开。第30天管腔的大小（直径）从p.n.第10天至p.n.第30天开始迅速增加，直到大约p.n.第50天早在p.n.就检测到曲细精管中的液体流动。第20天，之后数量增加。肌样细胞肌动蛋白丝束，平行运行，存在于p. n。第10天肌动蛋白在p.n.或稍早于p.n.时形成了一种成人特有的网状结构。第二十二天。这些数据表明，支持细胞内的肌动蛋白细胞骨架的发展和支持细胞屏障的初始形成之间存在时间关系。类似地，肌样细胞的肌动蛋白细胞骨架的发育与肾小管流体流动之间存在时间关系。然而，管腔直径的快速增加与Sertoli和肌样细胞骨架元件的初始发育无关。
The postnatal development of the Sertoli cell barrier, tubular lumen, fluid flow, and cytoskeletal elements in Sertoli and myoid cells was investigated in the Sprague-Dawley rat. With the aid of hypertonic fixatives, a barrier to the rapid entry of fluid was noted in the majority of tubules on the 15th and 16th postnatal (p.n.) days and was completely formed in all tubules prior to p.n. day 18. The actin forming the ectoplasmic specialization (ES), a cytoskeletal complex related to the occluding junctions composing the barrier, began its development during the period of initial barrier formation (16 p.n. day) and progressively attained its adult prominence. The ES developed its characteristic adult pattern and adult fluorescent intensity at about p.n. day 22. Some seminiferous tubules showed very small lumina as early as p.n. day 10. All tubules were not open until p.n. day 30. The size (diameter) of the lumen increased slowly from p.n. day 10 until p.n. day 30 when it started to increase rapidly until about p.n. day 50. Fluid flow in seminiferous tubules was detected as early as p.n. day 20 and increased in amount thereafter. Myoid cell actin filament bundles, running in parallel, were present at p.n. day 10. Actin formed a meshwork pattern characteristic of the adult on, or slightly prior to, p.n. day 22. These data indicate that there is a temporal relationship between the development of the actin cytoskeleton within the Sertoli cell and initial formation of the Sertoli cell barrier. Similarly, there is a temporal relationship between the development of the actin cytoskeleton of myoid cells and tubular fluid flow. The rapid increase in tubular lumen diameter, however, does not correlate with the initial development of Sertoli and myoid cytoskeletal elements.