Temporal and spatial changes in gene expression, metabolite accumulation and phytohormone content in rice seedlings grown under drought stress conditions-猫眼课题宝

DOI：

10.1111/tpj.13468

发表时间：

2017-04

期刊：

The Plant Journal

影响因子：

通讯作者：

中科院分区：

其他

文献类型：

作者：

D. Todaka;Yu Zhao;T. Yoshida;Madoka Kudo;Satoshi Kidokoro;J. Mizoi;Ken-Suke Kodaira;Yumiko Takebayashi;M. Kojima;H. Sakakibara;K. Toyooka;Mayuko Sato;A. Fernie;K. Shinozaki;K. Yamaguchi-Shinozaki

为了分析植物对不同程度干旱胁迫响应的分子机制，我们开发了一种基于土壤基质势（SMP）的灌溉系统，精确控制土壤水分。使用该系统，水稻幼苗在三个不同的干旱水平下生长，表示为Md 1，Md 2和Md 3，SMP值分别设置为-9.8，-31.0和-309.9 kPa。虽然Md 1治疗没有改变可见的表型，Md 2治疗引起气孔关闭和芽生长迟缓（SGR）。Md 3处理显著诱导SGR，而不抑制光合作用。更严重的干旱（Sds）处理，在此情况下，灌溉被终止，导致叶片萎蔫和光合作用的抑制。代谢组学分析显示，在Md 3和Sds下主要糖积累，在Sds下大多数氨基酸积累。Md 3处理使淀粉含量增加，Sds处理使淀粉含量降低。转录组数据显示，相关基因的表达谱支持所观察到的光合作用和代谢产物的变化，这表明从SGR到光合作用抑制的时间滞后可能导致Md 3下的光合产物的积累，其可以用作Sds下的渗透调节剂。为了进一步了解观察到的SGR，在特定组织中进行转录组和激素单体分析。结果表明，在Md 2、Md 3和Sds处理的茎基中，IAA和细胞分裂素的含量均明显降低，但IAA和细胞分裂素含量并不反映激素代谢相关基因的表达水平。这些结果表明，干旱胁迫影响细胞分裂素和IAA分子的分布或降解。

In order to analyze the molecular mechanisms underlying the responses of plants to different levels of drought stress, we developed a soil matric potential (SMP)-based irrigation system that precisely controls soil moisture. Using this system, rice seedlings were grown under three different drought levels, denoted Md1, Md2 and Md3, with SMP values set to -9.8, -31.0 and -309.9 kPa, respectively. Although the Md1 treatment did not alter the visible phenotype, the Md2 treatment caused stomatal closure and shoot growth retardation (SGR). The Md3 treatment markedly induced SGR, without inhibition of photosynthesis. More severe drought (Sds) treatment, under which irrigation was terminated, resulted in the wilting of leaves and inhibition of photosynthesis. Metabolome analysis revealed the accumulation of primary sugars under Md3 and Sds and of most amino acids under Sds. The starch content was increased under Md3 and decreased under Sds. Transcriptome data showed that the expression profiles of associated genes supported the observed changes in photosynthesis and metabolites, suggesting that the time lag from SGR to inhibition of photosynthesis might lead to the accumulation of photosynthates under Md3, which can be used as osmolytes under Sds. To gain further insight into the observed SGR, transcriptome and hormonome analyses were performed in specific tissues. The results showed specific decreases in indole-3-acetic acid (IAA) and cytokinin levels in Md2-, Md3- and Sds-treated shoot bases, though the expression levels of hormone metabolism-related genes were not reflected in IAA and cytokinin contents. These observations suggest that drought stress affects the distribution or degradation of cytokinin and IAA molecules.

Temporal and spatial changes in gene expression, metabolite accumulation and phytohormone content in rice seedlings grown under drought stress conditions

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