Growth and the Environment of Schizosaccharomyces pombe.
Growth and the Environment of Schizosaccharomyces pombe.
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DOI：
10.1101/pdb.top079764
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发表时间：
2016-03-01
影响因子：
--
通讯作者：
Russell, Paul
中科院分区：
文献类型：
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作者：
Petersen, Janni;Russell, Paul
在这里，我们总结了粟酒裂殖酵母培养基的组成和用途，并讨论了粟酒裂殖酵母培养物产生中需要考虑的关键问题。我们讨论了“培养记忆”的概念，其中菌株在储存、繁殖和发酵剂制备过程中经历的生长状态和应激可以改变后期的实验结果。我们还描述了广泛用于通过环境传感路径操纵信号的触发器。
Here, we summarize the composition and uses of Schizosaccharomyces pombe media and discuss key issues for consideration in the generation of S. pombe cultures. We discuss the concept of "culture memory," in which the growth state and stress experienced by a strain during storage, propagation, and starter culture preparation can alter experimental outcomes at later stages. We also describe the triggers that are widely used to manipulate signaling through the environment sensing pathways.