OsCOL16, encoding a CONSTANS-like protein, represses flowering by up regulating Ghd7 expression in rice
OsCOL16, encoding a CONSTANS-like protein, represses flowering by up regulating Ghd7 expression in rice
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OsCOL16 编码 CONSTANS 样蛋白，通过上调水稻中 Ghd7 的表达来抑制开花
DOI：
10.1016/j.plantsci.2017.04.004
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发表时间：
2017-07-01
期刊：
影响因子：
5.2
通讯作者：
Cheng, Shihua
中科院分区：
文献类型：
--
作者：
Wu, Weixun;Zheng, Xiao-Ming;Cheng, Shihua
开花期是协调植物生活史和区域适应性的重要农艺性状，从而影响谷类作物的产量潜力。CONSTANS（CO）-like基因家族在植物开花时间的调控中起着重要的作用。CO样蛋白在水稻中通常分为四个系统发育组。来自组I、III和IV的几个基因已经被功能性地表征，尽管对水稻中组II的基因知之甚少。我们报告的功能特性在水稻的组成型成花抑制剂，OsCOL 16，编码一组II CO样蛋白，延迟开花时间，增加株高和粮食产量。OsCOL 16的过量表达导致长日和短日条件下的抽穗延迟。OsCOL 16的表达呈现昼夜振荡，是一种具有转录激活活性的转录因子。我们确定OsCOL 16上调花阻遏物Ghd 7的表达，导致Ehd 1，Hd 3a和RFT 1的表达下调。此外，遗传多样性和进化分析表明，显着的开花时间的差异与OsCOL 16的两个主要等位基因。结合分子生物学和地理信息学分析，我们发现OsCOL 16在调节水稻光周期开花中发挥重要作用，从而使水稻能够适应环境。
Flowering time is an important agronomic trait that coordinates the plant life cycle with regional adaptability and thereby impacts yield potentials for cereal crops. The CONSTANS (CO)-like gene family plays vital roles in the regulation of flowering time. CO-like proteins are typically divided into four phylogenetic groups in rice. Several genes from groups I, III, and IV have been functionally characterized, though little is known about the genes of group II in rice. We report the functional characterization in rice of a constitutive floral inhibitor, OsCOL16, encoding a group-II CO-like protein that delays flowering time and increases plant height and grain yield. Overexpression of OsCOL16 resulted in late heading under both long-day and short-day conditions. OsCOL16 expression exhibits a diurnal oscillation and serves as a transcription factor with transcriptional activation activity. We determined that OsCOL16 up-regulates the expression of the floral repressor Ghd7, leading to down-regulation of the expression of Ehd1, Hd3a, and RFT1. Moreover, genetic diversity and evolutionary analyses suggest that remarkable differences in flowering times correlate with two major alleles of OsCOL16. Our combined molecular biology and phylogeographic analyses revealed that OsCOL16 plays an important role in regulating rice photoperiodic flowering, allowing for environmental adaptation of rice.