Ubiquitin-immunohistochemical investigation of atypical Pick's disease without Pick bodies
Ubiquitin-immunohistochemical investigation of atypical Pick's disease without Pick bodies
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DOI：
10.1016/s0022-510x(98)00168-3
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发表时间：
1998-08-14
影响因子：
4.4
通讯作者：
Kato, M
中科院分区：
文献类型：
--
作者：
Iseki, E;Li, F;Kato, M
本文报告6例非典型Pick病（PD）无Pick小体（PB）的免疫组化检查。这些病例表现出严重的神经元丢失，神经胶质增生主要发生在颞叶皮质。泛素免疫组化显示泛素阳性神经元内包涵体在齿状回和泛素阳性神经突起在大脑皮层。在齿状回，分子层内的树突和颗粒细胞内的神经元内包涵体呈阳性染色。免疫电镜下，两种结构均由泛素阳性的核糖体样颗粒成分和少数丝状成分组成。在大脑皮质中，泛素阳性神经突分布在II-IIIab层和V-VI层，被认为是小神经元的远端树突。这些神经元的树突和胞体含有类似于齿状回的泛素阳性成分。在海马下托、杏仁核和纹状体也发现了一些泛素阳性神经突起。本研究结果表明，齿状回颗粒细胞和大脑皮质小神经元在胞体和树突上具有共同的泛素相关和核糖体相关异常，海马旁损伤引起的穿通通路变性参与了颗粒细胞的泛素相关异常，PD伴和不伴PB的病例具有共同的受累神经元，如化学计量学所示。(C)1998 Elsevier Science B. V.保留所有权利。
Six cases of atypical Pick's disease (PD) without Pick bodies (PB) were examined immunohistochemically. These cases showed severe neuronal loss with gliosis predominantly in the temporal cortices. Ubiquitin immunohistochemistry revealed ubiquitin-positive intraneuronal inclusions in the dentate gyrus and ubiquitin-positive neurites in the cerebral cortex. In the dentate gyrus, the dendrites in the stratum moleculare as well as the intraneuronal inclusions in the granular cells were positively stained. Both structures were composed of ubiquitin-positive ribosome-like granular components and a few filamentous components immunoelectron-microscopically. In the cerebral cortex, ubiquitin-positive neurites were distributed in layers II-IIIab and layers V-VI, and were considered to be the distal dendrites from the small neurons. The dendrites and perikarya of these neurons contained ubiquitin-positive components similar to those in the dentate gyrus. Some ubiquitin-positive neurites were also found in the hippocampal subiculum, amygdala and striatum. The results of this study suggest that the granular cells in the dentate gyrus and the small neurons in the cerebral cortex share common ubiquitin-related and ribosome-associated abnormalities in both the perikarya and dendrites, that the degeneration of the perforant pathway caused by the parahippocampal lesion participates in the ubiquitin related abnormalities in the granular cells, and that PD cases with and without PB have common affected neurons, as shown immunohistochemically. (C) 1998 Elsevier Science B.V. All rights reserved.