Effect of bone-like layer growth from culture medium on adherence of osteoblast-like cells.
Effect of bone-like layer growth from culture medium on adherence of osteoblast-like cells.
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DOI：
10.1016/s0142-9612(02)00430-1
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发表时间：
2003-03
期刊：
影响因子：
14
通讯作者：
T. Kizuki;M. Ohgaki;Mihoko Katsura;Satoshi Nakamura;K. Hashimoto;Y. Toda;S. Udagawa;K. Yamashita
中科院分区：
文献类型：
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作者：
T. Kizuki;M. Ohgaki;Mihoko Katsura;Satoshi Nakamura;K. Hashimoto;Y. Toda;S. Udagawa;K. Yamashita
陶瓷HAp的电极化对加速骨修复有影响。研究了类骨生长层中的细胞行为。通过浸泡在补充有10%胎牛血清的α-最低必需培养基（补充有10%FBS的α-MEM）中，陶瓷HAp上的沉积物生长并形成层。随着生长层的生长，生长层上的贴壁细胞形状逐渐由纺锤形变为扁平形。在添加有10％FBS的α-MEM中浸泡7天在陶瓷HAp上生长的完全生长层上，所有粘附的细胞都是平坦的并且表面充满了生长的细胞。从这些结果可以看出，陶瓷HAp上的生长层是细胞生长的激活因素之一。因此，通过加速极化陶瓷 HAp 带负电的表面上的层生长，细胞生长得到加强。
The electrical polarization of ceramic HAp had an effect on the acceleration of bone restoration. Cell behavior in the bone-like growth layer was investigated. The deposits on the ceramic HAp was grown and formed layers by soaking in α-minimum essential medium supplemented with 10% fetal bovine serum (α-MEM supplemented with 10% FBS). The shapes of the adhering cells on the grown layer gradually changed from spindle to flat with growth of the layer. On the totally grown layer that was grown on the ceramic HAp by soaking in α-MEM supplemented with 10% FBS for 7 days, all the adhering cells were flat and the surface was filled with the grown cells. From these results, it was revealed that the grown layer on the ceramic HAp is one of the activation factors of cell growth. Consequently, cell growth was reinforced by acceleration of the layer growth on the negatively charged surface of the polarized ceramic HAp.