Diagnosing Treponema pallidum in secondary syphilis by PCR and immunohistochemistry
Diagnosing Treponema pallidum in secondary syphilis by PCR and immunohistochemistry
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DOI：
10.1038/sj.jid.5700888
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发表时间：
2007-10-01
影响因子：
6.5
通讯作者：
Dupin, Nicolas
中科院分区：
文献类型：
--
作者：
Buffet, Marc;Grange, Philippe A.;Dupin, Nicolas
在西方国家，梅毒的流行病学方面在病例数量方面发生了重大变化。梅毒螺旋体的检测很困难，二期梅毒的正确诊断可能是至关重要的。本研究使用了12例二期梅毒患者皮损的活检标本。梅毒的诊断是基于临床表现、暗视野显微镜分析和血清学测试。使用针对梅毒螺旋体的多克隆抗体，我们发现90%的样本中存在梅毒螺旋体，这些细菌位于表皮和真皮上部。梅毒螺旋体47 kDa表面蛋白基因在75%的皮损中可被扩增。当结合两种技术时，来自二期梅毒患者的样本中92%检测到梅毒螺旋体，但在对照样本中没有检测到梅毒螺旋体。我们的工作表明，免疫组织化学和聚合酶链式反应对二期梅毒的诊断都是有用的，在某些罕见的病例中，当血清学检测不出梅毒抗体时，免疫组织化学和聚合酶链式反应可能会有帮助。
Epidemiological aspects of syphilis in Western countries have undergone a significant change with respect to the number of cases. Detection of Treponema pallidum is difficult, and the correct diagnosis of secondary syphilis can be critical. In this study, biopsy samples from skin lesions of 12 patients with secondary syphilis were used. Diagnosis of syphilis was based on clinical presentation, dark- field microscope analysis, and serological tests. Using a polyclonal antibody directed against T. pallidum, we show the presence of T. pallidum in 90% of the samples studied with the bacteria located in the epidermis and the upper dermis. The T. pallidum 47- kDa surface protein gene could be amplified by PCR in 75% of the skin lesions. When combining both techniques, T. pallidum was detected in 92% of the samples from patients with secondary syphilis but not in the control samples. Our work suggests that both immunohistochemistry and PCR could be useful for the diagnosis of secondary syphilis and may be helpful in some rare cases when serological assays failed to detect T. pallidum antibodies.