Sodium and chloride transport across the isolated porcine gallbladder.
Sodium and chloride transport across the isolated porcine gallbladder.
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钠和氯通过离体猪胆囊运输。
DOI：
10.1152/ajpcell.1989.257.1.c45
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Wolters,PJ
中科院分区：
文献类型：
--
作者：
O'Grady,SM;Wolters,PJ
猪胆囊，剥离浆膜肌，安装在尤斯室中，并浸入等离子林格溶液中，产生 4-7 mV 的浆膜正跨上皮电位和 50-120 microA/cm2 的短路电流 (Isc)。用葡萄糖酸盐替代 Cl 或用 N-2-羟乙基哌嗪-N'-2-乙磺酸 (HEPES) 替代 HCO3 会导致 Isc 降低 50%。用 1 mM 阿米洛利（粘膜侧）或 0.1 mM 乙酰唑胺（两侧）治疗可抑制 Isc 25-27%。粘膜添加 4,4'-二异硫氰芪-2,2'-二磺酸可抑制 Isc 17%。浆膜中添加 0.1 mM 布美他尼可抑制 Isc 28%。阿米洛利 (1 mM) 分别抑制 Na 和 Cl 的净跨上皮通量 55% 和 41%。用葡萄糖酸盐替代 Cl 会抑制 50% 的净 Na 通量，而用 HEPES 替代 HCO3 会抑制 85-90% 的净 Na 通量，并将 Cl 吸收更改为净分泌。基于这些结果，假设 Na 和 Cl 穿过顶膜的转运是由两条途径介导的：Na-H/Cl-HCO3 交换和 Na-HCO3 共转运。 Cl 和 HCO3 的部分回收可能分别通过顶膜中的 Cl 传导途径和 Cl-HCO3 交换发生。这导致 Na 的净吸收，占基础条件下观察到的 Isc 的大部分。布美他尼对基底外侧膜的影响以及当 HCO3 不存在时发生 Cl 分泌的事实表明 Cl 分泌涉及与顶膜中 Cl 传导途径串联排列的基底外侧 NaCl 或 Na-K-Cl 共转运系统。
Porcine gallbladder, stripped of serosal muscle, mounted in Ussing chambers, and bathed in plasma-like Ringer solution generates a serosal positive transepithelial potential of 4-7 mV and a short-circuit current (Isc) of 50-120 microA/cm2. Substitution of Cl with gluconate or HCO3 with N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) results in a 50% decrease in Isc. Treatment with 1 mM amiloride (mucosal side) or 0.1 mM acetazolamide (both sides) causes 25-27% inhibition of the Isc. Mucosal addition of 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid inhibits the Isc by 17%. Serosal addition of 0.1 mM bumetanide inhibits the Isc by 28%. Amiloride (1 mM) inhibits the net transepithelial fluxes of Na and Cl by 55 and 41%, respectively. Substitution of Cl with gluconate inhibits the net Na flux by 50%, whereas substitution of HCO3 with HEPES inhibits 85-90% of the net Na flux and changes Cl absorption to net secretion. Based on these results, it is hypothesized that Na and Cl transport across the apical membrane is mediated by two pathways, Na-H/Cl-HCO3 exchange and Na-HCO3 cotransport. Partial recycling of Cl and HCO3 presumably occurs through a Cl conductive pathway and Cl-HCO3 exchange, respectively, in the apical membrane. This results in net Na absorption, which accounts for most of the Isc observed under basal conditions. The effect of bumetanide on the basolateral membrane and the fact that Cl secretion occurs when HCO3 is absent suggests that Cl secretion involves a basolateral NaCl or Na-K-Cl cotransport system arranged in series with a Cl conductive pathway in the apical membrane.