STAT5a regulates the GlcNAc-1-phosphate transferase gene transcription and expression.
STAT5a regulates the GlcNAc-1-phosphate transferase gene transcription and expression.
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STAT5a 调节 GlcNAc-1-磷酸转移酶基因的转录和表达。
DOI：
10.1159/000070252
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Vijay,InderK
中科院分区：
文献类型：
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作者：
Zhang,Xiao-Lian;Qu,Xue-Ju;Vijay,InderK
小鼠中的长叶醇-磷酸α-N-乙酰葡糖胺磷酸转移酶2（Dpagt 2）基因具有管家启动子，并且其表达在乳腺的发育过程中受到调节，并通过尿道调节泌乳。先前的研究表明，小鼠mammaryDpagt 2基因的转录受到催乳激素、胰岛素、糖皮质激素受体（GR）和催乳素的刺激。与Dpagt 2基因启动子区结合的转录因子可以影响Dpagt 2基因的表达水平。推测Dpagt 2基因启动子区（碱基对-1462 ~-5）可能含有10个STAT结合位点：TTN（5/6）AA。为了鉴定参与GPT基因转录的STAT因子，制备了32 P标记探针和泌乳小鼠核提取物。电泳迁移率改变分析（EMSA）显示该区域（碱基对-386至-322，其中存在STAT结合位点TTTCAAAAA）结合STAT 5a，而不结合糖皮质激素受体（GR）或其他STAT因子。通过瞬时转染不同的Dpagt 2启动子/荧光素酶（Luc）构建体到COS 7细胞中，进一步研究了STAT 5a参与调节小鼠Dpagt 2基因的表达。结果表明，共转染STAT 5a或催乳素受体可提高启动子pGL-MX6中Dpagt 2启动子的活性（从-386到-5），但对启动子pGL-MX7中Dpagt 2启动子的活性无明显影响（从-322到-5）。本文首次报道了STAT 5a参与Dpagt 2基因启动子-386和-322bp之间的结合，并刺激Dpagt 2基因在小鼠泌乳期乳腺中的转录表达。
The dolichyl-phosphate alpha-N-acetylglucosaminephosphotransferase 2 (Dpagt2) gene in the mouse has a housekeeping promoter, and its expression is regulated during the development and hormonally modulated lactogenesis of the mammary gland. Previous studies showed that the transcription of the mouse mammaryDpagt2 gene is stimulated by the lactogenic hormones, insulin, glucocorticoid receptor (GR), and prolactin. Transcription factors which bind to theDpagt2 gene promoter region can influence the expression level of theDpagt2 gene. It is supposed that theDpagt2 gene promoter region (bases pairs -1462 to -5) maybe contain 10 putative STAT (signal transducer and activator of transcription) binding sites: TTN(5/6)AA. In order to identify the STAT factors involved in the transcription of the GPT gene,32P labeling probes and lactating mouse nuclear extracts were prepared. Electrophoretic Mobility Shift Assays (EMSA) show that the region (bases pairs -386 to -322, where there is a STAT binding site, TTTCAAAAA) binds to STAT5a, not to glucocorticoid receptor (GR) or other STAT factors. The involvement of STAT5a in regulating the expression of the mouseDpagt2 gene was further investigated by transient transfections of variousDpagt2 promoter/luciferase (Luc) constructs into COS 7 cells. The results showed that co-transfection of STAT5a or prolactin receptor can enhanceDpagt2 promoter activities in the promoter construct pGL-MX6 (from base pairs -386 to -5), but not in the promoter construct pGL-MX7 (from base pairs -322 to -5). This paper first reports that STAT5a is involved in the binding between -386 and -322 base pairs of theDpagt2 gene promoter and stimulates the expression of theDpagt2 gene transcription in the mouse lactating mammary gland.