Biological and biochemical studies of African green monkey lymphotropic papovavirus
Biological and biochemical studies of African green monkey lymphotropic papovavirus
复制标题
非洲绿猴嗜淋巴细胞乳头多空病毒的生物学和生化研究
作者：
K. Takemoto;A. Furuno;K. Kato;K. Yoshiike
非洲绿色猴嗜淋巴乳头状空泡病毒（LPV）在人淋巴母细胞样细胞系BJA-B中的生长被发现是缓慢和低效的，这是由于缺陷颗粒的积累。对分子克隆的LPV DNA的分析表明，19个克隆中有3个克隆的DNA比其他克隆的DNA长（5.1倍酶）。5.1-脱氢酶DNA对BJA-B细胞具有感染性，而较短的（4.8-脱氢酶）分子则有缺陷。与野生型病毒不同，由克隆的感染性DNA制成的LPV原液是同质的，并且具有更高的滴度。使用无缺陷的LPV储备，我们研究了其他生物学特性。在另一种人B淋巴母细胞系中观察到LPV复制。该病毒接种到新生仓鼠体内时没有引起肿瘤。对人和非人灵长类动物血清的血清学调查表明，几乎所有灵长类动物，包括人，都有感染LPV抗原相关病毒的证据。
The growth of African green monkey lymphotropic papovavirus (LPV) in human lymphoblastoid cell line BJA-B was found to be slow and inefficient due to the accumulation of defective particles. An analysis of molecularly cloned LPV DNAs showed that 3 of 19 clones had DNAs that were longer (5.1 kilobases) than the DNAs of the other clones. The 5.1-kilobase DNA was infectious for BJA-B cells, whereas the shorter (4.8-kilobase) molecules were defective. Unlike the wild-type virus, stocks of LPV made from cloned, infectious DNAs were homogeneous and had higher titers. Using stocks of nondefective LPV, we investigated other biological properties. LPV replication in another human B-lymphoblastoid cell line was observed. The virus did not cause tumors when it was inoculated into newborn hamsters. Serological surveys of human and nonhuman primate sera indicated that virtually all primates, including humans, show evidence of infection by viruses antigenically related to LPV.