BACTERIOPHAGE PHI-6 - LIPID-CONTAINING VIRUS OF PSEUDOMONAS-PHASEOLICOLA
BACTERIOPHAGE PHI-6 - LIPID-CONTAINING VIRUS OF PSEUDOMONAS-PHASEOLICOLA
复制标题
DOI：
10.1128/jvi.11.5.799-805.1973
复制
发表时间：
1973-01-01
影响因子：
5.4
通讯作者：
VANETTEN, JL
中科院分区：
文献类型：
--
作者：
VIDAVER, AK;KOSKI, RK;VANETTEN, JL
本文报道了一种含脂质噬菌体φ6的纯化和性质。噬菌体含有一个脂质包膜，这可能是感染所必需的。在有机溶剂、脱氧胆酸钠和磷脂酶A的存在下，φ6的结晶性丧失。噬菌体脂质的脂肪酸组成与菜豆假单胞菌宿主细胞的脂肪酸组成相似。噬菌体由约25%的脂质、13%的RNA和62%的蛋白质组成。φ6在氯化铯中的浮密度为1.27g/ml。φ6的形态是不寻常的;它有一个约60 nm的多面体头部，周围有一个膜状的可压缩包膜，附着在皮利上时呈细长构型。吸附速率常数在半合成培养基中为3.3 × 10− 10 ml/min，在营养肉汤-酵母提取物培养基中为3.8 × 10− 10 ml/min。前者的潜伏期较短（80-115 min），而后者为120-160 min;平均爆发量较大（250-400），前者为125-150。日食期与潜伏期重合。
The purification and properties of a lipid-containing bacteriophage, φ6, are described. The phage contains a lipid envelope which is probably essential for infection. Infectivity of φ6 was lost in the presence of organic solvents, sodium deoxycholate, and phospholipase A. The fatty acid composition of the phage lipid was similar to that of thePseudomonas phaseolicolahost cells. The phage was composed of about 25% lipid, 13% RNA, and 62% protein. The buoyant density of φ6 was 1.27 g/ml in cesium chloride. The morphology of φ6 was unusual; it had a polyhedral head of about 60 nm surrounded by a membranous, compressible envelope which appeared to assume an elongated configuration upon attachment to pili. The adsorption rate constant was 3.3 × 10−10ml/min in a semi-synthetic medium and 3.8 × 10−10ml/min in a nutrient broth-yeast extract medium. The latent period was shorter in the former medium (80-115 min compared with 120-160 min), and the average burst size was larger (250-400 compared with 125-150). The eclipse period coincided with the latent period.