Tapered Side-Polished Microfibre Sensor for High Sensitivity hCG Detection
Tapered Side-Polished Microfibre Sensor for High Sensitivity hCG Detection
复制标题
用于高灵敏度 hCG 检测的锥形侧面抛光微纤维传感器
DOI：
10.1109/jsen.2022.3156284
复制
发表时间：
2022-04-15
影响因子：
4.3
通讯作者：
Wu, Qiang
中科院分区：
文献类型：
--
作者：
Luo, Wei;Liu, Bin;Wu, Qiang
A high sensitivity human chorionic gonado-tropin (hCG) detection was conducted by a tapered side-polished (TSP) optical fiber sensor. Experimentally, the TSP fiber sensor was made by side polishing a short section of single mode fiber to a D shape structure and tapering the D shape section to a small diameter (<inline-formula> <tex-math notation="LaTeX">$ < 10~\mu \text{m}$ </tex-math></inline-formula> in the experiments). By functionalizing the primary antibody of hCG onto the TSP fiber surface, the sensor was used for detecting hCG concentration. Experimental results show that when the hCG concentration is 0.1 mIU/mL, the sensor has an average wavelength shift of 0.82 nm. The limit of detection (LoD) of the hCG is estimated 0.058 mIU/mL, assuming three times of maximum wavelength variation (<inline-formula> <tex-math notation="LaTeX">${3}\times {0}.{15}=0.45$ </tex-math></inline-formula> nm) in Phosphate buffer saline (PBS) to the measurement limit. The specificity has also been tested by immersing the sensor into a mixed biomaterial solution (hCG - 1 mIU/mL, pig-IgG - 1 <inline-formula> <tex-math notation="LaTeX">$\mu \text{g}$ </tex-math></inline-formula>/mL, Staphylococcus aureus <inline-formula> <tex-math notation="LaTeX">$-{6}\times {10}^{{5}}$ </tex-math></inline-formula> CFU/mL and Escherichia coli - <inline-formula> <tex-math notation="LaTeX">${2.5}\times {10}^{{5}}$ </tex-math></inline-formula> CFU/mL). The result showed that the TSP optical fiber sensor has excellent specificity. The biosensor has potential application in clinical/medical diagnostics, human health, environmental quality and food safety monitoring.
A high sensitivity human chorionic gonado-tropin (hCG) detection was conducted by a tapered side-polished (TSP) optical fiber sensor. Experimentally, the TSP fiber sensor was made by side polishing a short section of single mode fiber to a D shape structure and tapering the D shape section to a small diameter (<inline-formula> <tex-math notation="LaTeX">$ < 10~\mu \text{m}$ </tex-math></inline-formula> in the experiments). By functionalizing the primary antibody of hCG onto the TSP fiber surface, the sensor was used for detecting hCG concentration. Experimental results show that when the hCG concentration is 0.1 mIU/mL, the sensor has an average wavelength shift of 0.82 nm. The limit of detection (LoD) of the hCG is estimated 0.058 mIU/mL, assuming three times of maximum wavelength variation (<inline-formula> <tex-math notation="LaTeX">${3}\times {0}.{15}=0.45$ </tex-math></inline-formula> nm) in Phosphate buffer saline (PBS) to the measurement limit. The specificity has also been tested by immersing the sensor into a mixed biomaterial solution (hCG - 1 mIU/mL, pig-IgG - 1 <inline-formula> <tex-math notation="LaTeX">$\mu \text{g}$ </tex-math></inline-formula>/mL, Staphylococcus aureus <inline-formula> <tex-math notation="LaTeX">$-{6}\times {10}^{{5}}$ </tex-math></inline-formula> CFU/mL and Escherichia coli - <inline-formula> <tex-math notation="LaTeX">${2.5}\times {10}^{{5}}$ </tex-math></inline-formula> CFU/mL). The result showed that the TSP optical fiber sensor has excellent specificity. The biosensor has potential application in clinical/medical diagnostics, human health, environmental quality and food safety monitoring.