Effect of crystalline phases of titania nanotube arrays on adipose derived stem cell adhesion and proliferation
Effect of crystalline phases of titania nanotube arrays on adipose derived stem cell adhesion and proliferation
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DOI：
10.1016/j.msec.2019.109850
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发表时间：
2019-10-01
影响因子：
7.9
通讯作者：
Soares, Paulo
中科院分区：
文献类型：
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作者：
Dias-Netipanyj, Marcela Ferreira;Cowden, Kari;Soares, Paulo
这项工作的目的是评估细胞对具有可变晶体结构的钛纳米管阵列的反应。评价了二氧化钛纳米管阵列的细胞毒性、活力和刺激脂肪来源的干细胞（ADSC）的粘附和增殖的能力。在二甘醇/氢氟酸电解液中，采用电化学阳极氧化法在60 V下氧化钛6 h，然后在300、530和630 ℃下退火5 h，制备了二氧化钛纳米管阵列。采用扫描电子显微镜（SEM）、接触角测定法、X射线衍射（XRD）和蛋白质吸附法对纳米管阵列进行了表征。将ADSC以1 × 10（4）个细胞/ml的密度培养在二氧化钛纳米管阵列上。使细胞粘附并增殖1、4和7天。通过CellTiter-Blue（R）细胞活力测定表征细胞活力;并且通过SEM表征细胞形态。通过用DAPI和罗丹明/鬼笔环肽染色细胞，使用荧光显微镜表征细胞粘附、增殖和形态。研究结果表明，在300 ℃和530 ℃退火时形成了金红石相，630 ℃退火时形成了金红石/金红石相。结果表明，二氧化钛纳米管阵列的晶体结构（即锐钛矿/金红石相）的修改影响ADSC的粘附和增殖。未来的研究将致力于评估这种细胞模型在成骨细胞中的分化。
The aim of this work was to evaluate the cellular response to titanium nanotube arrays with variable crystalline structure. Cytotoxicity, viability and the ability of the titania nanotube arrays to stimulate adhesion and proliferation of adipose derived stem cells (ADSCs) was evaluated. Titania nanotube arrays were fabricated by electrochemical anodization of titanium in diethyleneglycol/hydrofluoric acid electrolyte at 60 V for 6 h, then annealed at 300, 530 and 630 degrees C for 5 h. The nanotube arrays were characterized using scanning electron microscopy (SEM), contact angle goniometry, x-ray diffraction (XRD) and protein adsorption. ADSCs were cultured on titania nanotube arrays at a density of 1 x 10(4) cells/ml. The cells were allowed to adhere and to proliferate for 1, 4 and 7 days. Cell viability was characterized by the CellTiter-Blue (R) Cell Viability Assay; and cell morphology was characterized by SEM. Cell adhesion, proliferation and morphology were characterized using fluorescence microscopy by staining the cells with DAPI and rhodamine/phalloidin. The results from this study showed that the annealing at 300 and 530 degrees C formed anatase phase, and annealing at 630 degrees C formed anatase/rutile phase. The results indicated that the modification of the crystalline structure (i.e. anatase/rutile phase) of titania nanotube arrays influenced the ADSC adhesion and proliferation. Future studies are now directed towards evaluating differentiation of this cellular model in osteoblasts.