Inhibition of starfish oocyte maturation by some inhibitors of proteolytic enzymes
Inhibition of starfish oocyte maturation by some inhibitors of proteolytic enzymes
复制标题
一些蛋白水解酶抑制剂对海星卵母细胞成熟的抑制作用
DOI：
10.1002/mrd.1120050103
复制
发表时间：
1982
期刊：
影响因子：
--
通讯作者：
H. Kanatani
中科院分区：
文献类型：
--
作者：
T. Kishimoto;T. Clark;H. Kondo;H. Shirai;H. Kanatani
Starfish oocyte maturation is triggered by a natural hormone, 1-methyladenine (1-MeAde), produced in the follicle cells, or artificially by dithiothreitol (DTT). These substances act on the oocyte surface to produce a cytoplasmic maturation-promoting factor (MPF), which induces germinal vesicle breakdown (GVBD) and subsequent processes of meiotic maturation. Further, MPF is amplified in immature oocytes that have received the injection of MPF. In this paper the effect of leupeptin and antipain, protease inhibitors of microbial origin, on starfish oocyte maturation was investigated. The protease inhibitors were found to inhibit 1-MeAde-induced maturation when they were applied externally or injected into oocytes. DTT-induced maturation was also inhibited by injection of leupeptin. However, leupeptin did not inhibit the maturation-inducing action of MPF or MPF amplification. These results show that the protease inhibitors suppress the production of MPF by 1-MeAde or DTT, suggesting that some endogenous protease(s) acts in the production of MPF.
