A ROLE FOR EXON SEQUENCES AND SPLICE-SITE PROXIMITY IN SPLICE-SITE SELECTION
A ROLE FOR EXON SEQUENCES AND SPLICE-SITE PROXIMITY IN SPLICE-SITE SELECTION
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DOI：
10.1016/0092-8674(86)90343-0
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发表时间：
1986-08-29
期刊：
影响因子：
64.5
通讯作者：
MANIATIS, T
中科院分区：
文献类型：
--
作者：
REED, R;MANIATIS, T
含有人β-半乳糖苷酶的5“或3”剪接位点的串联重复的RNA前体的体外剪接产物的分析珠蛋白IVS 1揭示外显子序列在重复位点的相对使用中起重要作用。这些研究还表明，5“和3”剪接位点的接近度是选择剪接位点的重要决定因素。外显子序列的缺失、取代或甚至细微的变化都可以改变模式或剪接位点选择，并且在许多情况下，不使用与改变的外显子相邻的剪接位点。重复剪接位点的相对使用也可以通过稀释剪接提取物来改变，这表明剪接位点选择所涉及的因素是有限的。
Analysis of the in vitro splicing products of RNA precursors containing tandem duplications of the 5'' or 3'' splice sites of human .beta.-globin IVS 1 revealed that exon sequences play an important role in the relative use of the duplicated sites. These studies also show that the proximity of the 5'' and 3'' splice sites is an important determinant in the selection of splice-sites. Deletion, substitution, or even subtle changes of exon sequences can alter the pattern or splice-site selection, and in many cases the splice site adjacent to the altered exon is not used. The relative use of the duplicated splice sites can also be altered by diluting the splicing extract, suggesting that factors involved in splice-site selection are limiting.