MECHANOTRANSDUCTION IN BONE - DO BONE-CELLS ACT AS SENSORS OF FLUID-FLOW
MECHANOTRANSDUCTION IN BONE - DO BONE-CELLS ACT AS SENSORS OF FLUID-FLOW
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DOI：
10.1096/fasebj.8.11.8070637
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发表时间：
1994-08-01
期刊：
影响因子：
4.8
通讯作者：
OTTER, MW
中科院分区：
文献类型：
--
作者：
TURNER, CH;FORWOOD, MR;OTTER, MW
当密质骨受到弯曲载荷时，骨基质中的间质液从高压缩应力区域流出。间质液流量的量强烈影响的负荷率在剂量依赖性的方式。我们假设，间质液流影响骨形成，我们间接测试了这一假设，通过测量不同的负载频率对骨形成率在体内的影响。成年雌性大鼠的右胫骨受到施加弯曲的频率为0.05，0.1，0.2，0.5，1.0和2.0 Hz的2周的时间。然后处死大鼠，对胫骨中段进行骨形成的组织形态学测量。弯曲的胫骨增加骨形成率在较高的频率（0.5至2.0 Hz）的负载组多达四倍，但没有增加骨形成率的负载频率低于0.5 Hz。在一个单独的实验中，我们发现在大鼠胫骨的应力产生的电位（SGP）单调增加，随着负载频率的增加。加载频率和骨形成反应之间的剂量-反应关系与加载频率和骨内SGP之间的关系非常相似。这两种关系之间的定性相似性表明，骨形成增加与SGP增加有关，这是由间质液流动引起的。已知骨细胞对电场敏感，并且可直接响应SGP。此外，流体剪切力已被证明可以刺激培养中的骨细胞，因此增加的间质液流动可能直接影响骨形成。
When compact bone is subjected to bending loads, interstitial fluid in the bone matrix flows away from regions of high compressive stress. The amount of interstitial fluid flow is strongly influenced by the loading rate in a dose-dependent fashion. We hypothesize that interstitial fluid flow affects bone formation, and we tested this hypothesis indirectly by measuring the effect of different loading frequencies on bone formation rate in vivo. The right tibiae of adult female rats were subjected to applied bending at frequencies of 0.05, 0.1, 0.2, 0.5, 1.0, and 2.0 Hz for a 2-wk period. The rats were then killed and histomorphometric measurements of bone formation were made of the midshaft of the tibia. Bending of the tibia increased bone formation rate in the higher-frequency (0.5 to 2.0 Hz) loading groups as much as fourfold, yet no increase in bone formation rate was observed for loading frequencies below 0.5 Hz. In a separate experiment, we found stress-generated potentials (SGP) in the rat tibia to increase monotonically with increasing loading frequency. The dose-response relationship between loading frequency and the bone formation response closely resembles the relationship between loading frequency and SGP within bone. The qualitative similarity between these two relationships suggests that increased bone formation is associated with increased SGP, which are caused by interstitial fluid flow. Bone cells are known to be sensitive to electric fields and may respond directly to SGP. Also, fluid shear forces have been shown to stimulate bone cells in culture, so it is possible that increased interstitial fluid flow directly affects bone formation.