Using Heterogeneous Stocks for Fine-Mapping Genetically Complex Traits.
Using Heterogeneous Stocks for Fine-Mapping Genetically Complex Traits.
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使用异质种群精细绘制复杂的遗传性状。
DOI：
10.1007/978-1-4939-9581-3_11
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发表时间：
2019
期刊：
影响因子：
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通讯作者：
Palmer,AbrahamA
中科院分区：
文献类型：
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作者：
SolbergWoods,LeahC;Palmer,AbrahamA
在这一章中，我们将回顾利用异质群体(HS)群体在小鼠和大鼠身上精细定位复杂性状的原理和实验设计。我们将HS定义为由两个或多个近交系杂交而来的异交群体。HS已被用于对多种行为、生理和基因表达性状进行全基因组关联研究。使用HS的GWAS需要四个关键步骤，我们回顾一下：选择合适的HS人群，表型分析，基因分型和统计分析。我们提供了选择HS的建议，评论了与表型相关的关键问题，讨论了与这些群体相关的基因分型方法，并描述了适用于使用HS的遗传分析的统计遗传分析。
In this chapter we will review both the rationale and experimental design for using Heterogeneous Stock (HS) populations for fine-mapping of complex traits in mice and rats. We define an HS as an outbred population derived from an intercross between two or more inbred strains. HS have been used to perform genome-wide association studies (GWAS) for multiple behavioral, physiological, and gene expression traits. GWAS using HS require four key steps, which we review: selection of an appropriate HS population, phenotyping, genotyping, and statistical analysis. We provide advice on the selection of an HS, comment on key issues related to phenotyping, discuss genotyping methods relevant to these populations, and describe statistical genetic analyses that are applicable to genetic analyses that use HS.