Laser-guidance-based cell deposition microscope for heterotypic single-cell micropatterning.
Laser-guidance-based cell deposition microscope for heterotypic single-cell micropatterning.
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DOI：
10.1088/1758-5082/3/3/034107
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发表时间：
2011-09
期刊：
影响因子：
9
通讯作者：
Gao BZ
中科院分区：
文献类型：
--
作者：
Ma Z;Pirlo RK;Wan Q;Yun JX;Yuan X;Xiang P;Borg TK;Gao BZ
细胞图案化方法使研究人员能够控制特定的同型和异型接触介导的细胞-细胞和细胞-ECM相互作用，并施加限定的细胞和组织几何形状。为了将单个细胞微图案化到具有高空间分辨率的基底上的特定点，我们开发了一种基于激光引导技术的细胞沉积显微镜。我们讨论了产生激光制导的光学力的理论和优化的光学配置（NA = 0.1），以操纵细胞的高速在三维空间。我们的细胞沉积显微镜能够将不同类型的细胞图案化到标准细胞研究设备上和设备内，并为长期细胞培养提供台上孵育。使用该细胞沉积显微镜，将大鼠骨髓间充质干细胞与心肌细胞一起微图案化到22 mm × 22 mm盖玻片上的聚二甲基硅氧烷微结构基底上，形成单细胞共培养微环境，观察共培养过程中细胞电生理特性的变化。
Cell patterning methods enable researchers to control specific homotypic and heterotypic contact-mediated cell-cell and cell-ECM interactions and to impose defined cell and tissue geometries. To micropattern individual cells to specific points on a substrate with high spatial resolution, we have developed a cell deposition microscope based on the laser guidance technique. We discuss the theory of optical forces for generating laser guidance and the optimization of the optical configuration (NA ≈ 0.1) to manipulate cells with high speed in three dimensions. Our cell deposition microscope is capable of patterning different cell types onto and within standard cell research devices and providing on-stage incubation for long-term cell culturing. Using this cell deposition microscope, rat mesenchymal stem cells from bone marrow were micropatterned with cardiomyocytes into a substrate microfabricated with polydimethylsiloxane on a 22mm × 22 mm coverglass to form a single-cell coculturing microenvironment, and their electrophysiological property changes were investigated during the coculturing days.