Genomic and phylogenetic analyses of murine adenovirus 2
Genomic and phylogenetic analyses of murine adenovirus 2
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DOI：
10.1016/j.virusres.2011.05.023
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发表时间：
2011-09-01
期刊：
影响因子：
5
通讯作者：
Harrach, Balazs
中科院分区：
文献类型：
--
作者：
Hemmi, Silvio;Vidovszky, Marton Z.;Harrach, Balazs
鼠腺病毒（MAdV）被认为是乳腺病毒属最古老的成员。目前，已知三种不同的 MAdV 类型具有相当不同的趋向性和病理学。在这里，我们报告并注释了 MAdV-2 全基因组的 DNA 序列。结果发现，它由 35,203 bp 组成，因此比其他两种 MAdV 类型的基因组大得多。 MAdV-2基因组大小的增加通常是由于较大的基因和ORF，尽管在早期区域E1、E3和E4中观察到ORF的数量存在一些差异。 MAdV-2 的 El B 19K 基因的同源物编码 330 个氨基酸 (aa)，几乎是其他乳腺病毒的两倍。因此，只有 N 端一半（155 个氨基酸）与 19K 蛋白具有同源性。在 MAdV-2 的 E3 区域中鉴定出了 12.5K 蛋白基因的同源物，但在 MAdV-1 或 MAdV-3 中则没有。 MAdV-2 E3 区域中另一个功能未知的基因似乎是独特的。 MAdV-2 的 E4 区域包含三个 ORF。其中一种与其他 AdV 的 34K 基因相似。 MAdV-2 E4 区中的两个独特的 ORF 分别与 MAdV-1 或 MAdV-3 E4 区中的五个和六个 ORF 没有同源性。系统发育分析表明，这三种鼠类 AdV 具有密切的共同祖先。它们可能形成了乳腺病毒谱系的第一个分支，并且似乎是该属最古老的代表。 (C) 2011 Elsevier B.V. 保留所有权利。
Murine adenoviruses (MAdV) are supposedly the oldest members of the genus Mastadenovirus. Currently, there are three distinct MAdV types known with rather different tropism and pathology. Here we report and annotate the DNA sequence of the full genome of MAdV-2. It was found to consist of 35,203 bp thus being considerably larger than the genomes of the other two MAdV types. The increased size of the MAdV-2 genome is generally due to larger genes and ORFs, although some differences in the number of ORFs were observed for the early regions El, E3 and E4. The homologue of the 19K gene of El B from MAdV-2 codes for 330 amino acids (aa) and is almost twice as large as from other mastadenoviruses. Accordingly, only the N-terminal half (155 aa) has homology to the 19K protein. A homologue of the gene of the 12.5K protein was identified in the E3 region of MAdV-2, but not in MAdV-1 or MAdV-3. The other gene of yet unknown function in the E3 region of MAdV-2 seems to be unique. The E4 region of MAdV-2 contains three ORFs. One has similarity to the 34K gene of other AdVs. Two unique ORFs in the E4 region of MAdV-2 have no homology to any of the five and six ORFs in the E4 region of MAdV-1 or MAdV-3, respectively. Phylogenetic analyses showed that the three murine AdVs have a close common ancestor. They likely formed the first branching of the lineage of mastadenoviruses, and seem to be the most ancient representatives of this genus. (C) 2011 Elsevier B.V. All rights reserved.