Spontaneous inflammatory bowel disease in multiple mutant mouse lines: Association with colonization by Helicobacter hepaticus
Spontaneous inflammatory bowel disease in multiple mutant mouse lines: Association with colonization by Helicobacter hepaticus
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DOI：
10.1046/j.1523-5378.1998.08006.x
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发表时间：
1998-06-01
期刊：
影响因子：
4.4
通讯作者：
Schauer, DB
中科院分区：
文献类型：
--
作者：
Foltz, CJ;Fox, JG;Schauer, DB
背景资料。遗传和微生物因素都被认为在炎症性肠病(IBD)的发展中起作用：然而，还没有明确定义人类或动物的致病微生物。正常的菌群或以前未知的肠道病原体可能会导致易感宿主的疾病发展。一种新发现的小鼠幽门螺杆菌，肝病螺旋体，会导致小鼠肝炎，其中一种小鼠与肝癌有关。本研究探讨多个基因改变的肝型肝炎小鼠下肠道定植与IBD发生的关系。材料和方法。临床上在多个基因改变的小鼠品系中发现的直肠脱垂，评估了H型肝炎病毒的存在和IBD的组织学证据。对代表11种不同基因改变的55只小鼠进行了评估。在患有直肠脱垂的突变小鼠中，85%的小鼠检出了肝包虫。65%的动物有增殖性伤寒、结肠炎或直肠炎的组织学证据，其中89%的动物经种特异性PCR检测为肝炎型H。在多个基因改变的小鼠中存在与IBD相关的肝型肝炎病毒，这表明需要进一步的研究来从实验上测试肝型肝炎病毒在易感小鼠的IBD发展中所起的作用。此外，本研究中感染肝片吸虫的特定突变小鼠株系可能为阐明IBD发病机制中的微生物和遗传因素提供额外的模型。
Background. Both genetic and microbial factors are thought to play a role in the development of inflammatory bowel disease (IBD): however, no causative microbial agent has been clearly defined for humans or animals. Normal flora or previously unrecognized intestinal pathogens may contribute to the development of disease in susceptible hosts. A newly recognized murine Helicobacter, H. hepaticus, causes hepatitis in mice and in one strain of mice is linked to liver cancer. This study investigates the association between colonization of the lower intestinal tract of multiple genetically altered lines of mice with H. hepaticus, and the occurrence of IBD.Materials and Methods. Rectal prolapse noted clinically in multiple genetically altered mouse lines was evaluated for the presence of H. hepaticus and histologic evidence of IBD. Fifty-five mice representing 11 different genetic alterations were evaluated.Results. H. hepaticus was detected in 85% of mutant mice with rectal prolapse. Histologic evidence of proliferative typhlitis, colitis or proctitis was present in 65% of the animals examined, 89% of which were positive for H. hepaticus as detected by species specific PCR.Conclusion. The presence of H. hepaticus in association with IBD in multiple lines of genetically altered mice suggests further studies are needed to test experimentally the role H. hepaticus plays in the development of IBD in susceptible mice. Additionally, specific mutant mouse lines infected with H. hepaticus is in this study may provide additional models for elucidation of microbial and genetic factors in the pathogenesis of IBD.