Altered tRNA methylation in rats and mice fed lipotrope-deficient diets.
Altered tRNA methylation in rats and mice fed lipotrope-deficient diets.
复制标题
喂食缺乏促脂剂的饮食的大鼠和小鼠的 tRNA 甲基化发生了改变。
DOI：
10.1093/carcin/7.3.473
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发表时间：
1986
期刊：
影响因子：
4.7
通讯作者：
M. Baliś
中科院分区：
文献类型：
--
作者：
E. Wainfan;M. Dizik;M. Hluboky;M. Baliś
在雄性Fischer大鼠中，发现缺乏蛋氨酸、胆碱、叶酸和维生素B12的饮食可迅速诱导肝脏tRNA甲基化的改变。体外试验表明，活性的N2-鸟嘌呤tRNA甲基转移酶II（NMG 2）增加到150%的控制水平，在1周和300%的控制水平后，2周或更长的时间，这种饮食。从这些动物的肝脏中分离出不完全甲基化的tRNA，表明体内甲基化受损。因此，这种甲基缺乏的饮食对肝脏tRNA甲基化的影响被认为是模仿肝脏致癌物乙醇的影响，乙醇也会导致低甲基化tRNA的产生和NMG 2活性的增加。研究了同一饲料对C57 BL/6 J和C3 H/HeJ近交系小鼠肝脏tRNA甲基转移酶活性的影响。摄入的脂代谢缺乏的饮食诱导升高的活性，肝脏N2-鸟嘌呤tRNA甲基转移酶II的活性在C57 BL/6 J小鼠，类似于在大鼠中看到的。相比之下，甲基缺乏的饮食对C3 H/HeJ动物相同的酶活性的影响很小。
A diet that is deficient in methionine, choline, folic acid and vitamin B12 has been found to induce alterations rapidly in liver tRNA methylation in male Fischer rats. In vitro assays indicated that activity of N2-guanine tRNA methyltransferase II (NMG2) was increased to 150% of controls levels in 1 week and 300% of control levels after 2 weeks or longer on this diet. Incompletely methylated tRNA was isolated from livers of these same animals, indicating that there was impairment of methylation in vivo. The effects on liver tRNA methylation of this methyl-deficient diet were thus seen to mimic those of the liver carcinogen, ethionine, which also causes production of hypomethylated tRNA and increased activity of NMG2. The effect of the same diet on liver tRNA methyltransferase activity of C57BL/6J and C3H/HeJ inbred mice were also studied. Intake of the lipotrope-deficient diet induced elevation in activity of liver N2-guanine tRNA methyltransferase II activity in C57BL/6J mice, similar to that seen in rats. In contrast, the methyl-deficient diet had very little effect on the same enzyme activity in C3H/HeJ animals.