Molecular characterization of S locus genes, SLG and SRK, in a pollen-recessive self-incompatibility haplotype of Brassica rapa L.
Molecular characterization of S locus genes, SLG and SRK, in a pollen-recessive self-incompatibility haplotype of Brassica rapa L.
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发表时间：
1998-07
期刊：
影响因子：
3.3
通讯作者：
K. Hatakeyama;T. Takasaki;M. Watanabe;K. Hinata
中科院分区：
文献类型：
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作者：
K. Hatakeyama;T. Takasaki;M. Watanabe;K. Hinata
在表现出自交不亲和性的芸苔属物种中，位于S位点的两个基因SLG和SRK参与了与自交和非自交花粉的识别反应。从芜菁的花粉隐性单倍型S29中，分离并鉴定了这两个基因的cDNA和基因组DNA克隆。SRK29的S结构域核苷酸序列与SLG29的S结构域核苷酸序列高度相似，属于II类。RNA凝胶印迹分析表明，SLG29的转录本由第一和第二外显子组成，没有检测到含有内含子序列任何部分的其他转录本。由于SLG29的第二外显子不编码跨膜结构域，因此SLG29被命名为分泌型糖蛋白。B的另外两个花粉隐性单倍型S40和S44的SLG。rapa也具有与SLG 29相似的结构。以前，SLG 2从花粉隐性单倍型，S2，甘蓝被发现产生两种不同的成绩单，一个为分泌型糖蛋白和其他假定的膜锚定形式的SLG。因此，这些SLG的性质来自花粉隐性单倍型B。rapa与B的SLG 2不同。甘蓝。
In Brassica species that exhibit self-incompatibility, two genes, SLG and SRK, at the S locus are involved in the recognition reaction with self and non-self pollen. From a pollen-recessive S29 haplotype of Brassica rapa, both cDNA and genomic DNA clones for these two genes were isolated and characterized. The nucleotide sequence for the S domain of SRK29 showed a high degree of similarity with that of SLG29, and they belong to Class II type. RNA gel blot analysis showed that the transcript of SLG29 consisted of the first and second exons, and no other transcript containing any part of the intron sequence was detected. Because no transmembrane domain was encoded by the second exon of SLG29, SLG29 was designated a secreted type glycoprotein. SLGs of two other pollen-recessive haplotypes, S40 and S44, of B. rapa also had a similar structure to that of SLG29. Previously, SLG2 from a pollen-recessive haplotype, S2, of Brassica oleracea was found to produce two different transcripts, one for the secreted type glycoprotein and the other for a putative membrane-anchored form of SLG. Therefore, the nature of these SLGs from pollen-recessive haplotypes of B. rapa is different from that of SLG2 of B. oleracea.