[Simultaneous determination of N-methylcarbamate pesticides and their metabolites in agricultural products].
[Simultaneous determination of N-methylcarbamate pesticides and their metabolites in agricultural products].
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农产品中N-甲基氨基甲酸酯类农药及其代谢物的同时测定[J].
DOI：
10.3358/shokueishi.43.133
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Kazuo Saito
中科院分区：
文献类型：
--
作者：
Maki Kobayashi;T. Nagayama;I. Takano;Yasuhiro Tamura;N. Kimura;K. Kitayama;Kazuo Saito
研究了固相萃取柱净化后高效液相色谱-柱后荧光检测法测定农产品中N-甲基氨基甲酸酯类农药及其代谢物的方法。用丙酮提取农产品匀浆。粗提物在5%氯化钠溶液和二氯甲烷中分配，二氯甲烷层浓缩至干燥。残留物用丙酮和正己烷的混合物溶解，并用Supellean ENVI-Carb SPE、Bond Elut萃取柱PSA和SAX与丙酮和正己烷的混合物串联进行纯化。采用高效液相色谱-柱后反应-荧光检测法对氨基甲酸甲酯农药进行分析。该方法可以准确分析柑橘类水果和各种农产品中的氨基甲酸甲酯农药。在几种农产品中添加0.10ppm的N-甲基氨基甲酸酯农药的回收率大多在60-110%之间。检出限为0.005 ppm。
We investigated the determination of N-methylcarbamate pesticides and their metabolites in agricultural products by HPLC with post-column fluorescence detection after clean-up with an SPE cartridge. The homogenate of agricultural products was extracted with acetone. The crude extract was partitioned between 5% sodium chloride solution and dichloromethane, and the dichloromethane layer was concentrated to dryness. The residue was dissolved with a mixture of acetone and n-hexane, and purified by using Supelclean ENVI-Carb SPE, Bond Elut Extraction Cartridge PSA and SAX in series with a mixture of acetone and n-hexane. N-Methylcarbamate pesticide was analyzed by HPLC with post-column reaction and fluoresce detection. N-Methylcarbamate pesticide in citrus fruits and various kinds of agricultural products could be analyzed accurately by the presented method. Recoveries of N-methylcarbamate pesticides added to several agricultural products at the level of 0.10 ppm were mostly in the range of 60-110%. The limit of detection was 0.005 ppm.